ssr_primer
Primer Details for TRINITY_DN78530_c2_g2_i1 |
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| (T)10ctttttttttc(T)10c(T)10: Primer 1 details |
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| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | TTTCATGCTTTCAGGGGCCT | TGCATTGCAAATTCCGCCAA | Start: 1735 |
| Tm | 59.885 | 59.966 | End: 1908 |
| Size | 20 | 20 | 174 |
| Primer 2 details |
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| Sequence | GTTGGACGGCCAGTACGTAA | AGGCCCCTGAAAGCATGAAA | Start: 1579 |
| Tm | 60.038 | 59.885 | End: 1754 |
| Size | 20 | 20 | 176 |
| Primer 3 details |
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| Sequence | ATAAAGGTGCGAGGCAGGAG | GAAGTCATGAGTCCCACGCA | Start: 109 |
| Tm | 59.821 | 60.037 | End: 246 |
| Size | 20 | 20 | 138 |
| Primer 4 details |
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| Sequence | ATAAAGGTGCGAGGCAGGAG | TAGCTCACCTCCCGAAGTCA | Start: 109 |
| Tm | 59.821 | 59.961 | End: 259 |
| Size | 20 | 20 | 151 |
| Primer 5 details |
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| Sequence | TCGTTGATAGTTGGACGGCC | AGGCCCCTGAAAGCATGAAA | Start: 1570 |
| Tm | 60.109 | 59.885 | End: 1754 |
| Size | 20 | 20 | 185 |
(G)12: Primer 1 details | | Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | TTTCATGCTTTCAGGGGCCT | TGCATTGCAAATTCCGCCAA | Start: 1735 |
| Tm | 59.885 | 59.966 | End: 1908 |
| Size | 20 | 20 | 174 |
| Primer 2 details |
|---|
| Sequence | GTTGGACGGCCAGTACGTAA | AGGCCCCTGAAAGCATGAAA | Start: 1579 |
| Tm | 60.038 | 59.885 | End: 1754 |
| Size | 20 | 20 | 176 |
| Primer 3 details |
|---|
| Sequence | ATAAAGGTGCGAGGCAGGAG | GAAGTCATGAGTCCCACGCA | Start: 109 |
| Tm | 59.821 | 60.037 | End: 246 |
| Size | 20 | 20 | 138 |
| Primer 4 details |
|---|
| Sequence | ATAAAGGTGCGAGGCAGGAG | TAGCTCACCTCCCGAAGTCA | Start: 109 |
| Tm | 59.821 | 59.961 | End: 259 |
| Size | 20 | 20 | 151 |
| Primer 5 details |
|---|
| Sequence | TCGTTGATAGTTGGACGGCC | AGGCCCCTGAAAGCATGAAA | Start: 1570 |
| Tm | 60.109 | 59.885 | End: 1754 |
| Size | 20 | 20 | 185 |
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