ssr_primer
Primer Details for TRINITY_DN78520_c0_g1_i1 |
|---|
| (T)18: Primer 1 details |
|---|
| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | AGGGACAATGCTTTACGCCA | CAAGCTTTTTCTGCCACCCC | Start: 822 |
| Tm | 59.962 | 59.966 | End: 991 |
| Size | 20 | 20 | 170 |
| Primer 2 details |
|---|
| Sequence | AGGGACAATGCTTTACGCCA | TGCAAGCTTTTTCTGCCACC | Start: 822 |
| Tm | 59.962 | 59.895 | End: 993 |
| Size | 20 | 20 | 172 |
| Primer 3 details |
|---|
| Sequence | ATACTTGTTTTGTGCGGGCG | TCAACGCGGCAACTGTAGTA | Start: 2233 |
| Tm | 59.76 | 59.685 | End: 2456 |
| Size | 20 | 20 | 224 |
| Primer 4 details |
|---|
| Sequence | ATACTTGTTTTGTGCGGGCG | ATTTTCAACGCGGCAACTGT | Start: 2233 |
| Tm | 59.76 | 59.616 | End: 2460 |
| Size | 20 | 20 | 228 |
| Primer 5 details |
|---|
| Sequence | AGGGACAATGCTTTACGCCA | GCAAGCTTTTTCTGCCACCC | Start: 822 |
| Tm | 59.962 | 60.601 | End: 992 |
| Size | 20 | 20 | 171 |
(T)10: Primer 1 details | | Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | AGGGACAATGCTTTACGCCA | CAAGCTTTTTCTGCCACCCC | Start: 822 |
| Tm | 59.962 | 59.966 | End: 991 |
| Size | 20 | 20 | 170 |
| Primer 2 details |
|---|
| Sequence | AGGGACAATGCTTTACGCCA | TGCAAGCTTTTTCTGCCACC | Start: 822 |
| Tm | 59.962 | 59.895 | End: 993 |
| Size | 20 | 20 | 172 |
| Primer 3 details |
|---|
| Sequence | ATACTTGTTTTGTGCGGGCG | TCAACGCGGCAACTGTAGTA | Start: 2233 |
| Tm | 59.76 | 59.685 | End: 2456 |
| Size | 20 | 20 | 224 |
| Primer 4 details |
|---|
| Sequence | ATACTTGTTTTGTGCGGGCG | ATTTTCAACGCGGCAACTGT | Start: 2233 |
| Tm | 59.76 | 59.616 | End: 2460 |
| Size | 20 | 20 | 228 |
| Primer 5 details |
|---|
| Sequence | AGGGACAATGCTTTACGCCA | GCAAGCTTTTTCTGCCACCC | Start: 822 |
| Tm | 59.962 | 60.601 | End: 992 |
| Size | 20 | 20 | 171 |
(T)10: Primer 1 details | | Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | AGGGACAATGCTTTACGCCA | CAAGCTTTTTCTGCCACCCC | Start: 822 |
| Tm | 59.962 | 59.966 | End: 991 |
| Size | 20 | 20 | 170 |
| Primer 2 details |
|---|
| Sequence | AGGGACAATGCTTTACGCCA | TGCAAGCTTTTTCTGCCACC | Start: 822 |
| Tm | 59.962 | 59.895 | End: 993 |
| Size | 20 | 20 | 172 |
| Primer 3 details |
|---|
| Sequence | ATACTTGTTTTGTGCGGGCG | TCAACGCGGCAACTGTAGTA | Start: 2233 |
| Tm | 59.76 | 59.685 | End: 2456 |
| Size | 20 | 20 | 224 |
| Primer 4 details |
|---|
| Sequence | ATACTTGTTTTGTGCGGGCG | ATTTTCAACGCGGCAACTGT | Start: 2233 |
| Tm | 59.76 | 59.616 | End: 2460 |
| Size | 20 | 20 | 228 |
| Primer 5 details |
|---|
| Sequence | AGGGACAATGCTTTACGCCA | GCAAGCTTTTTCTGCCACCC | Start: 822 |
| Tm | 59.962 | 60.601 | End: 992 |
| Size | 20 | 20 | 171 |
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