ssr_primer
Primer Details for TRINITY_DN78465_c2_g2_i2 |
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| (A)21: Primer 1 details |
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| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | CCAGGCCCCCTTCTCTTTTT | AGAAGTGGCGTTCTTCGGAG | Start: 133 |
| Tm | 59.885 | 60.038 | End: 352 |
| Size | 20 | 20 | 220 |
| Primer 2 details |
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| Sequence | CCAGGCCCCCTTCTCTTTTT | GAAGTGGCGTTCTTCGGAGA | Start: 133 |
| Tm | 59.885 | 60.039 | End: 351 |
| Size | 20 | 20 | 219 |
| Primer 3 details |
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| Sequence | GCGGCTTGAAGAGAGGGTAA | AGAAGTGGCGTTCTTCGGAG | Start: 241 |
| Tm | 59.751 | 60.038 | End: 352 |
| Size | 20 | 20 | 112 |
| Primer 4 details |
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| Sequence | GCGGCTTGAAGAGAGGGTAA | GAAGTGGCGTTCTTCGGAGA | Start: 241 |
| Tm | 59.751 | 60.039 | End: 351 |
| Size | 20 | 20 | 111 |
| Primer 5 details |
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| Sequence | AGAAAGAGGAGGTCCCCAGG | AGAAGTGGCGTTCTTCGGAG | Start: 118 |
| Tm | 60.253 | 60.038 | End: 352 |
| Size | 20 | 20 | 235 |
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