ssr_primer
Primer Details for TRINITY_DN78451_c2_g2_i3 |
|---|
| (A)10: Primer 1 details |
|---|
| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | TCAGACAACGGCCAACAACT | GGGTGAAATGCTCTGCTCCT | Start: 302 |
| Tm | 60.107 | 60.035 | End: 521 |
| Size | 20 | 20 | 220 |
| Primer 2 details |
|---|
| Sequence | TCATCAGACAACGGCCAACA | GGGTGAAATGCTCTGCTCCT | Start: 299 |
| Tm | 59.892 | 60.035 | End: 521 |
| Size | 20 | 20 | 223 |
| Primer 3 details |
|---|
| Sequence | GCGCAGATGAAAGGAGAGGT | GCCGAGCGATGACAGACTAA | Start: 458 |
| Tm | 60.108 | 59.899 | End: 584 |
| Size | 20 | 20 | 127 |
| Primer 4 details |
|---|
| Sequence | CTTTGCCTTGTGTGCTTGCT | GTGAGTCGTGTTCCCCCTTT | Start: 0 |
| Tm | 59.896 | 59.892 | End: 190 |
| Size | 20 | 20 | 191 |
| Primer 5 details |
|---|
| Sequence | AGGAGCAGAGCATTTCACCC | TCGGATTCTTGGCATCGCAT | Start: 502 |
| Tm | 60.035 | 60.179 | End: 679 |
| Size | 20 | 20 | 178 |
(A)33: Primer 1 details | | Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | TCAGACAACGGCCAACAACT | GGGTGAAATGCTCTGCTCCT | Start: 302 |
| Tm | 60.107 | 60.035 | End: 521 |
| Size | 20 | 20 | 220 |
| Primer 2 details |
|---|
| Sequence | TCATCAGACAACGGCCAACA | GGGTGAAATGCTCTGCTCCT | Start: 299 |
| Tm | 59.892 | 60.035 | End: 521 |
| Size | 20 | 20 | 223 |
| Primer 3 details |
|---|
| Sequence | GCGCAGATGAAAGGAGAGGT | GCCGAGCGATGACAGACTAA | Start: 458 |
| Tm | 60.108 | 59.899 | End: 584 |
| Size | 20 | 20 | 127 |
| Primer 4 details |
|---|
| Sequence | CTTTGCCTTGTGTGCTTGCT | GTGAGTCGTGTTCCCCCTTT | Start: 0 |
| Tm | 59.896 | 59.892 | End: 190 |
| Size | 20 | 20 | 191 |
| Primer 5 details |
|---|
| Sequence | AGGAGCAGAGCATTTCACCC | TCGGATTCTTGGCATCGCAT | Start: 502 |
| Tm | 60.035 | 60.179 | End: 679 |
| Size | 20 | 20 | 178 |
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