ssr_primer
Primer Details for TRINITY_DN78375_c2_g3_i3 |
|---|
| (T)11: Primer 1 details |
|---|
| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | TCCCCATAAGGCTGGAAGGT | GGCATGCGAAGTGTCAATGG | Start: 251 |
| Tm | 60.254 | 60.179 | End: 478 |
| Size | 20 | 20 | 228 |
| Primer 2 details |
|---|
| Sequence | TCCCCATAAGGCTGGAAGGT | GGGCATGCGAAGTGTCAATG | Start: 251 |
| Tm | 60.254 | 60.179 | End: 479 |
| Size | 20 | 20 | 229 |
| Primer 3 details |
|---|
| Sequence | GCGCCTCTCTCAAGATAGCC | GGCATGCGAAGTGTCAATGG | Start: 368 |
| Tm | 60.319 | 60.179 | End: 478 |
| Size | 20 | 20 | 111 |
| Primer 4 details |
|---|
| Sequence | GCGCCTCTCTCAAGATAGCC | GGGCATGCGAAGTGTCAATG | Start: 368 |
| Tm | 60.319 | 60.179 | End: 479 |
| Size | 20 | 20 | 112 |
| Primer 5 details |
|---|
| Sequence | TCCCCATAAGGCTGGAAGGT | AGAATCTGCAGCACTGAGGG | Start: 251 |
| Tm | 60.254 | 59.748 | End: 526 |
| Size | 20 | 20 | 276 |
(T)10: Primer 1 details | | Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | TCCCCATAAGGCTGGAAGGT | GGCATGCGAAGTGTCAATGG | Start: 251 |
| Tm | 60.254 | 60.179 | End: 478 |
| Size | 20 | 20 | 228 |
| Primer 2 details |
|---|
| Sequence | TCCCCATAAGGCTGGAAGGT | GGGCATGCGAAGTGTCAATG | Start: 251 |
| Tm | 60.254 | 60.179 | End: 479 |
| Size | 20 | 20 | 229 |
| Primer 3 details |
|---|
| Sequence | GCGCCTCTCTCAAGATAGCC | GGCATGCGAAGTGTCAATGG | Start: 368 |
| Tm | 60.319 | 60.179 | End: 478 |
| Size | 20 | 20 | 111 |
| Primer 4 details |
|---|
| Sequence | GCGCCTCTCTCAAGATAGCC | GGGCATGCGAAGTGTCAATG | Start: 368 |
| Tm | 60.319 | 60.179 | End: 479 |
| Size | 20 | 20 | 112 |
| Primer 5 details |
|---|
| Sequence | TCCCCATAAGGCTGGAAGGT | AGAATCTGCAGCACTGAGGG | Start: 251 |
| Tm | 60.254 | 59.748 | End: 526 |
| Size | 20 | 20 | 276 |
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