ssr_primer
Primer Details for TRINITY_DN78335_c1_g2_i2 |
|---|
| (T)10: Primer 1 details |
|---|
| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | ACACCATTACGCAACGTCCT | CTCTCCGCGTGGAACAGATT | Start: 110 |
| Tm | 59.966 | 60.109 | End: 290 |
| Size | 20 | 20 | 181 |
| Primer 2 details |
|---|
| Sequence | AATCTGTTCCACGCGGAGAG | TGACTTGCCGGGTTGCATAT | Start: 271 |
| Tm | 60.109 | 60.035 | End: 444 |
| Size | 20 | 20 | 174 |
| Primer 3 details |
|---|
| Sequence | AATCTGTTCCACGCGGAGAG | ATGACTTGCCGGGTTGCATA | Start: 271 |
| Tm | 60.109 | 60.035 | End: 445 |
| Size | 20 | 20 | 175 |
| Primer 4 details |
|---|
| Sequence | AATCTGTTCCACGCGGAGAG | ATAGATCAAGGCGCGACGTT | Start: 271 |
| Tm | 60.109 | 59.898 | End: 506 |
| Size | 20 | 20 | 236 |
| Primer 5 details |
|---|
| Sequence | AACGTCGCGCCTTGATCTAT | TAGCACGGCAGCTTACTGTC | Start: 487 |
| Tm | 59.898 | 60.109 | End: 693 |
| Size | 20 | 20 | 207 |
(T)14: Primer 1 details | | Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | ACACCATTACGCAACGTCCT | CTCTCCGCGTGGAACAGATT | Start: 110 |
| Tm | 59.966 | 60.109 | End: 290 |
| Size | 20 | 20 | 181 |
| Primer 2 details |
|---|
| Sequence | AATCTGTTCCACGCGGAGAG | TGACTTGCCGGGTTGCATAT | Start: 271 |
| Tm | 60.109 | 60.035 | End: 444 |
| Size | 20 | 20 | 174 |
| Primer 3 details |
|---|
| Sequence | AATCTGTTCCACGCGGAGAG | ATGACTTGCCGGGTTGCATA | Start: 271 |
| Tm | 60.109 | 60.035 | End: 445 |
| Size | 20 | 20 | 175 |
| Primer 4 details |
|---|
| Sequence | AATCTGTTCCACGCGGAGAG | ATAGATCAAGGCGCGACGTT | Start: 271 |
| Tm | 60.109 | 59.898 | End: 506 |
| Size | 20 | 20 | 236 |
| Primer 5 details |
|---|
| Sequence | AACGTCGCGCCTTGATCTAT | TAGCACGGCAGCTTACTGTC | Start: 487 |
| Tm | 59.898 | 60.109 | End: 693 |
| Size | 20 | 20 | 207 |
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