ssr_primer
Primer Details for TRINITY_DN78325_c3_g1_i2 |
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| (A)13: Primer 1 details |
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| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | AACACACGTTTCTGCATGGC | AGTGGTGAAGCAGCTGAACA | Start: 930 |
| Tm | 59.969 | 59.819 | End: 1030 |
| Size | 20 | 20 | 101 |
| Primer 2 details |
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| Sequence | TTGCACAGAAGAGGAGCTCC | TCTGCCTCAGCCCTTCATTG | Start: 1796 |
| Tm | 59.677 | 60.035 | End: 1925 |
| Size | 20 | 20 | 130 |
| Primer 3 details |
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| Sequence | TGCCCCTTTTATGCCTTCGA | GCCATGCAGAAACGTGTGTT | Start: 672 |
| Tm | 59.67 | 59.969 | End: 949 |
| Size | 20 | 20 | 278 |
| Primer 4 details |
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| Sequence | AGAGTCGCGTACGTTCAAGG | ATCAATAGGTGCCGTTGCCT | Start: 1859 |
| Tm | 60.11 | 59.744 | End: 1968 |
| Size | 20 | 20 | 110 |
| Primer 5 details |
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| Sequence | GAGTCGCGTACGTTCAAGGA | ATCAATAGGTGCCGTTGCCT | Start: 1860 |
| Tm | 60.11 | 59.744 | End: 1968 |
| Size | 20 | 20 | 109 |
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