ssr_primer
Primer Details for TRINITY_DN78308_c1_g2_i6 |
|---|
| (T)24: Primer 1 details |
|---|
| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | ACACTCCTGATACGCGCAAA | AAACATGCTGCGACTTGGTG | Start: 92 |
| Tm | 60.038 | 59.688 | End: 365 |
| Size | 20 | 20 | 274 |
| Primer 2 details |
|---|
| Sequence | ACACTCCTGATACGCGCAAA | CAAACATGCTGCGACTTGGT | Start: 92 |
| Tm | 60.038 | 59.688 | End: 366 |
| Size | 20 | 20 | 275 |
| Primer 3 details |
|---|
| Sequence | ACACTCCTGATACGCGCAAA | CTGCGACTTGGTGCACTTTT | Start: 92 |
| Tm | 60.038 | 59.618 | End: 358 |
| Size | 20 | 20 | 267 |
| Primer 4 details |
|---|
| Sequence | ACCAAGTCGCAGCATGTTTG | TAGAAATGCACCGAGTCGCA | Start: 347 |
| Tm | 59.688 | 59.755 | End: 563 |
| Size | 20 | 20 | 217 |
| Primer 5 details |
|---|
| Sequence | CACCAAGTCGCAGCATGTTT | TAGAAATGCACCGAGTCGCA | Start: 346 |
| Tm | 59.688 | 59.755 | End: 563 |
| Size | 20 | 20 | 218 |
(C)21tcccccccacaccaccactcccccaaccttcccttttttc(T)18: Primer 1 details | | Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | ACACTCCTGATACGCGCAAA | AAACATGCTGCGACTTGGTG | Start: 92 |
| Tm | 60.038 | 59.688 | End: 365 |
| Size | 20 | 20 | 274 |
| Primer 2 details |
|---|
| Sequence | ACACTCCTGATACGCGCAAA | CAAACATGCTGCGACTTGGT | Start: 92 |
| Tm | 60.038 | 59.688 | End: 366 |
| Size | 20 | 20 | 275 |
| Primer 3 details |
|---|
| Sequence | ACACTCCTGATACGCGCAAA | CTGCGACTTGGTGCACTTTT | Start: 92 |
| Tm | 60.038 | 59.618 | End: 358 |
| Size | 20 | 20 | 267 |
| Primer 4 details |
|---|
| Sequence | ACCAAGTCGCAGCATGTTTG | TAGAAATGCACCGAGTCGCA | Start: 347 |
| Tm | 59.688 | 59.755 | End: 563 |
| Size | 20 | 20 | 217 |
| Primer 5 details |
|---|
| Sequence | CACCAAGTCGCAGCATGTTT | TAGAAATGCACCGAGTCGCA | Start: 346 |
| Tm | 59.688 | 59.755 | End: 563 |
| Size | 20 | 20 | 218 |
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