Primer Details for TRINITY_DN78284_c5_g1_i1 | |||
|---|---|---|---|
| (A)23tgcacttacgcgccttgtcctcctcagtggcgtggcgggaatttcgatattttcatttttgtgccatttaaaaccct(A)11: Primer 1 details | |||
| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | AGCGAATTTTTCACAGAAAATGCA | CACTGAGGAGGACAAGGCG | Start: 2 |
| Tm | 58.951 | 60.08 | End: 117 |
| Size | 24 | 19 | 116 |
| Primer 2 details | |||
| Sequence | AGCGAATTTTTCACAGAAAATGCA | CGCCACTGAGGAGGACAAG | Start: 2 |
| Tm | 58.951 | 60.08 | End: 120 |
| Size | 24 | 19 | 119 |
| Primer 3 details | |||
| Sequence | AGCGAATTTTTCACAGAAAATGCA | GAGGACAAGGCGCGTAAGT | Start: 2 |
| Tm | 58.951 | 60.08 | End: 110 |
| Size | 24 | 19 | 109 |
| Primer 4 details | |||
| Sequence | AGCGAATTTTTCACAGAAAATGCA | TATCGAAATTCCCGCCACGC | Start: 2 |
| Tm | 58.951 | 61.155 | End: 137 |
| Size | 24 | 20 | 136 |
| Primer 5 details | |||
| Sequence | GAGCGAATTTTTCACAGAAAATGCA | CACTGAGGAGGACAAGGCG | Start: 1 |
| Tm | 59.825 | 60.08 | End: 117 |
| Size | 25 | 19 | 117 |
