ssr_primer
Primer Details for TRINITY_DN78272_c3_g2_i2 |
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| (A)12ccacaatactgacacgccagcttccatacttcctaacgatataacaagcataaaatggacaatgtggcccggcaactaaaaattaccagatg(A)10: Primer 1 details |
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| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | TGTGGAACGGTTTCAAGGGT | CACCTTGTCTCATGTCGGCT | Start: 84 |
| Tm | 59.744 | 60.037 | End: 277 |
| Size | 20 | 20 | 194 |
| Primer 2 details |
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| Sequence | TATGCTGCAGGCTTCCAGAG | GGCTCTCTCTGAGTCTTGCC | Start: 1923 |
| Tm | 59.82 | 59.824 | End: 2048 |
| Size | 20 | 20 | 126 |
| Primer 3 details |
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| Sequence | AGCCGACATGAGACAAGGTG | AGCCTTGCCTGATTCGTTCT | Start: 258 |
| Tm | 60.037 | 59.674 | End: 386 |
| Size | 20 | 20 | 129 |
| Primer 4 details |
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| Sequence | AGCCGACATGAGACAAGGTG | AGAGCCTTGCCTGATTCGTT | Start: 258 |
| Tm | 60.037 | 59.674 | End: 388 |
| Size | 20 | 20 | 131 |
| Primer 5 details |
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| Sequence | AACGAATCAGGCAAGGCTCT | AGCAACTGTCATCCCTGCAA | Start: 369 |
| Tm | 59.674 | 59.889 | End: 581 |
| Size | 20 | 20 | 213 |
(A)12: Primer 1 details | | Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | TGTGGAACGGTTTCAAGGGT | CACCTTGTCTCATGTCGGCT | Start: 84 |
| Tm | 59.744 | 60.037 | End: 277 |
| Size | 20 | 20 | 194 |
| Primer 2 details |
|---|
| Sequence | TATGCTGCAGGCTTCCAGAG | GGCTCTCTCTGAGTCTTGCC | Start: 1923 |
| Tm | 59.82 | 59.824 | End: 2048 |
| Size | 20 | 20 | 126 |
| Primer 3 details |
|---|
| Sequence | AGCCGACATGAGACAAGGTG | AGCCTTGCCTGATTCGTTCT | Start: 258 |
| Tm | 60.037 | 59.674 | End: 386 |
| Size | 20 | 20 | 129 |
| Primer 4 details |
|---|
| Sequence | AGCCGACATGAGACAAGGTG | AGAGCCTTGCCTGATTCGTT | Start: 258 |
| Tm | 60.037 | 59.674 | End: 388 |
| Size | 20 | 20 | 131 |
| Primer 5 details |
|---|
| Sequence | AACGAATCAGGCAAGGCTCT | AGCAACTGTCATCCCTGCAA | Start: 369 |
| Tm | 59.674 | 59.889 | End: 581 |
| Size | 20 | 20 | 213 |
(A)20: Primer 1 details | | Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | TGTGGAACGGTTTCAAGGGT | CACCTTGTCTCATGTCGGCT | Start: 84 |
| Tm | 59.744 | 60.037 | End: 277 |
| Size | 20 | 20 | 194 |
| Primer 2 details |
|---|
| Sequence | TATGCTGCAGGCTTCCAGAG | GGCTCTCTCTGAGTCTTGCC | Start: 1923 |
| Tm | 59.82 | 59.824 | End: 2048 |
| Size | 20 | 20 | 126 |
| Primer 3 details |
|---|
| Sequence | AGCCGACATGAGACAAGGTG | AGCCTTGCCTGATTCGTTCT | Start: 258 |
| Tm | 60.037 | 59.674 | End: 386 |
| Size | 20 | 20 | 129 |
| Primer 4 details |
|---|
| Sequence | AGCCGACATGAGACAAGGTG | AGAGCCTTGCCTGATTCGTT | Start: 258 |
| Tm | 60.037 | 59.674 | End: 388 |
| Size | 20 | 20 | 131 |
| Primer 5 details |
|---|
| Sequence | AACGAATCAGGCAAGGCTCT | AGCAACTGTCATCCCTGCAA | Start: 369 |
| Tm | 59.674 | 59.889 | End: 581 |
| Size | 20 | 20 | 213 |
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