ssr_primer
Primer Details for TRINITY_DN78270_c4_g1_i4 |
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| (T)21(G)10tgggt(G)36: Primer 1 details |
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| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | TCCTCTATAAGCCGCACCCT | AACACCCACGGTAATGCGAT | Start: 448 |
| Tm | 60.106 | 60.036 | End: 719 |
| Size | 20 | 20 | 272 |
| Primer 2 details |
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| Sequence | TCCTCTATAAGCCGCACCCT | TCTCTAGACACCACGGCTGA | Start: 448 |
| Tm | 60.106 | 59.962 | End: 683 |
| Size | 20 | 20 | 236 |
| Primer 3 details |
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| Sequence | GCAACTTTAAAGGCCCGTCG | AGGGTGCGGCTTATAGAGGA | Start: 316 |
| Tm | 60.11 | 60.106 | End: 467 |
| Size | 20 | 20 | 152 |
| Primer 4 details |
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| Sequence | AAGGTGGAGGATGGGGTACA | CATTTAGAGGTCCCGACGGG | Start: 184 |
| Tm | 59.88 | 59.896 | End: 348 |
| Size | 20 | 20 | 165 |
| Primer 5 details |
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| Sequence | AAGGTGGAGGATGGGGTACA | CGACGGGCCTTTAAAGTTGC | Start: 184 |
| Tm | 59.88 | 60.11 | End: 335 |
| Size | 20 | 20 | 152 |
(A)20: Primer 1 details | | Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | TCCTCTATAAGCCGCACCCT | AACACCCACGGTAATGCGAT | Start: 448 |
| Tm | 60.106 | 60.036 | End: 719 |
| Size | 20 | 20 | 272 |
| Primer 2 details |
|---|
| Sequence | TCCTCTATAAGCCGCACCCT | TCTCTAGACACCACGGCTGA | Start: 448 |
| Tm | 60.106 | 59.962 | End: 683 |
| Size | 20 | 20 | 236 |
| Primer 3 details |
|---|
| Sequence | GCAACTTTAAAGGCCCGTCG | AGGGTGCGGCTTATAGAGGA | Start: 316 |
| Tm | 60.11 | 60.106 | End: 467 |
| Size | 20 | 20 | 152 |
| Primer 4 details |
|---|
| Sequence | AAGGTGGAGGATGGGGTACA | CATTTAGAGGTCCCGACGGG | Start: 184 |
| Tm | 59.88 | 59.896 | End: 348 |
| Size | 20 | 20 | 165 |
| Primer 5 details |
|---|
| Sequence | AAGGTGGAGGATGGGGTACA | CGACGGGCCTTTAAAGTTGC | Start: 184 |
| Tm | 59.88 | 60.11 | End: 335 |
| Size | 20 | 20 | 152 |
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