Primer Details for TRINITY_DN78233_c1_g1_i1 |
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| (T)21: Primer 1 details |
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| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | TGATACAAATGTTGATGTGCATGA | GCCGGTGCCAGTTTTATAGG | Start: 102 |
| Tm | 57.488 | 58.978 | End: 212 |
| Size | 24 | 20 | 111 |
| Primer 2 details |
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| Sequence | TGATACAAATGTTGATGTGCATGA | GCCGGTGCCAGTTTTATAGGA | Start: 102 |
| Tm | 57.488 | 60.408 | End: 212 |
| Size | 24 | 21 | 111 |
| Primer 3 details |
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| Sequence | TGATACAAATGTTGATGTGCATGAA | GCCGGTGCCAGTTTTATAGG | Start: 102 |
| Tm | 58.078 | 58.978 | End: 212 |
| Size | 25 | 20 | 111 |
| Primer 4 details |
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| Sequence | TTGATACAAATGTTGATGTGCATGA | GCCGGTGCCAGTTTTATAGG | Start: 101 |
| Tm | 58.078 | 58.978 | End: 212 |
| Size | 25 | 20 | 112 |
| Primer 5 details |
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| Sequence | ACAATTGATACAAATGTTGATGTGCA | GCCGGTGCCAGTTTTATAGG | Start: 97 |
| Tm | 58.847 | 58.978 | End: 212 |
| Size | 26 | 20 | 116 |
