Primer Details for TRINITY_DN78165_c4_g1_i1 | |||
|---|---|---|---|
| (T)11g(T)23: Primer 1 details | |||
| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | TTGGAATTGTAAGTGGAGTAGCGA | CTTCGTGAATGGATCCTTTTGAGG | Start: 30 |
| Tm | 60.021 | 59.905 | End: 129 |
| Size | 24 | 24 | 100 |
| Primer 2 details | |||
| Sequence | TGGAATTGTAAGTGGAGTAGCGA | ACTTCGTGAATGGATCCTTTTGA | Start: 31 |
| Tm | 59.488 | 58.348 | End: 130 |
| Size | 23 | 23 | 100 |
| Primer 3 details | |||
| Sequence | TGGAATTGTAAGTGGAGTAGCGA | ACTTCGTGAATGGATCCTTTTGAG | Start: 31 |
| Tm | 59.488 | 59.303 | End: 130 |
| Size | 23 | 24 | 100 |
| Primer 4 details | |||
| Sequence | ACATTGGAATTGTAAGTGGAGTAGC | CGTGAATGGATCCTTTTGAGGG | Start: 27 |
| Tm | 59.352 | 59.315 | End: 126 |
| Size | 25 | 22 | 100 |
| Primer 5 details | |||
| Sequence | TTGGAATTGTAAGTGGAGTAGCG | CTTCGTGAATGGATCCTTTTGAGG | Start: 30 |
| Tm | 58.75 | 59.905 | End: 129 |
| Size | 23 | 24 | 100 |
