ssr_primer
Primer Details for TRINITY_DN78141_c0_g2_i3 |
|---|
| (T)19: Primer 1 details |
|---|
| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | GGATAAGCGCCGTTTCGTTC | AAGGACCATCCCCTCATCCA | Start: 1477 |
| Tm | 59.974 | 59.954 | End: 1708 |
| Size | 20 | 20 | 232 |
| Primer 2 details |
|---|
| Sequence | CTCCTTCGGGGGATACTGGA | ATTGCCAGGAGCTGCAGATT | Start: 674 |
| Tm | 60.106 | 60.033 | End: 820 |
| Size | 20 | 20 | 147 |
| Primer 3 details |
|---|
| Sequence | TCTCCTTCGGGGGATACTGG | ATTGCCAGGAGCTGCAGATT | Start: 673 |
| Tm | 60.106 | 60.033 | End: 820 |
| Size | 20 | 20 | 148 |
| Primer 4 details |
|---|
| Sequence | GTTCGCCGGAAATCGTGAAG | AAGGACCATCCCCTCATCCA | Start: 1493 |
| Tm | 59.905 | 59.954 | End: 1708 |
| Size | 20 | 20 | 216 |
| Primer 5 details |
|---|
| Sequence | TGGATGAGGGGATGGTCCTT | TGGCAACCCTGTTGAACCAT | Start: 1689 |
| Tm | 59.954 | 60.106 | End: 1809 |
| Size | 20 | 20 | 121 |
(A)18: Primer 1 details | | Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | GGATAAGCGCCGTTTCGTTC | AAGGACCATCCCCTCATCCA | Start: 1477 |
| Tm | 59.974 | 59.954 | End: 1708 |
| Size | 20 | 20 | 232 |
| Primer 2 details |
|---|
| Sequence | CTCCTTCGGGGGATACTGGA | ATTGCCAGGAGCTGCAGATT | Start: 674 |
| Tm | 60.106 | 60.033 | End: 820 |
| Size | 20 | 20 | 147 |
| Primer 3 details |
|---|
| Sequence | TCTCCTTCGGGGGATACTGG | ATTGCCAGGAGCTGCAGATT | Start: 673 |
| Tm | 60.106 | 60.033 | End: 820 |
| Size | 20 | 20 | 148 |
| Primer 4 details |
|---|
| Sequence | GTTCGCCGGAAATCGTGAAG | AAGGACCATCCCCTCATCCA | Start: 1493 |
| Tm | 59.905 | 59.954 | End: 1708 |
| Size | 20 | 20 | 216 |
| Primer 5 details |
|---|
| Sequence | TGGATGAGGGGATGGTCCTT | TGGCAACCCTGTTGAACCAT | Start: 1689 |
| Tm | 59.954 | 60.106 | End: 1809 |
| Size | 20 | 20 | 121 |
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