ssr_primer
Primer Details for TRINITY_DN78127_c4_g4_i2 |
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| (T)16: Primer 1 details |
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| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | CCTCAGCTTCCCTCTCTCCT | GGGGTAGAGTACTCGGGTGT | Start: 2501 |
| Tm | 60.032 | 60.034 | End: 2758 |
| Size | 20 | 20 | 258 |
| Primer 2 details |
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| Sequence | ATCCAGACCTCAGCTTCCCT | GGGGTAGAGTACTCGGGTGT | Start: 2494 |
| Tm | 59.956 | 60.034 | End: 2758 |
| Size | 20 | 20 | 265 |
| Primer 3 details |
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| Sequence | ACACCCGAGTACTCTACCCC | TCCCAGTCTGGTTGAGCAAC | Start: 2739 |
| Tm | 60.034 | 59.891 | End: 2842 |
| Size | 20 | 20 | 104 |
| Primer 4 details |
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| Sequence | AGCACTCGACCTGTAACTGC | GCTCTCGTGTGCTCTCCAAT | Start: 78 |
| Tm | 60.038 | 60.108 | End: 319 |
| Size | 20 | 20 | 242 |
| Primer 5 details |
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| Sequence | TCAGACCTTGCCTCCTCTCA | GGGGTAGAGTACTCGGGTGT | Start: 2543 |
| Tm | 59.885 | 60.034 | End: 2758 |
| Size | 20 | 20 | 216 |
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