ssr_primer
Primer Details for TRINITY_DN78065_c4_g3_i2 |
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| (A)46: Primer 1 details |
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| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | GGCGGTATACCACGACTGAG | GACCAGTGGACAGGTGACAG | Start: 193 |
| Tm | 59.97 | 59.966 | End: 357 |
| Size | 20 | 20 | 165 |
| Primer 2 details |
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| Sequence | ACCTTGGGAGCACTCTTTCG | GGCCATGGCGTTTTTGTCTT | Start: 1239 |
| Tm | 59.965 | 59.967 | End: 1512 |
| Size | 20 | 20 | 274 |
| Primer 3 details |
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| Sequence | ACCTTGGGAGCACTCTTTCG | ACCAGGGCAAATTTTTCCGC | Start: 1239 |
| Tm | 59.965 | 59.965 | End: 1462 |
| Size | 20 | 20 | 224 |
| Primer 4 details |
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| Sequence | AAGACAAAAACGCCATGGCC | CAGAACTTGCGCAGGTTGAC | Start: 1493 |
| Tm | 59.967 | 60.041 | End: 1732 |
| Size | 20 | 20 | 240 |
| Primer 5 details |
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| Sequence | ACTGTCCCTCCCTACGAGTC | GCGCAAAGTTTCTAGAGCGG | Start: 1989 |
| Tm | 60.034 | 59.905 | End: 2168 |
| Size | 20 | 20 | 180 |
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