ssr_primer
Primer Details for TRINITY_DN78042_c4_g1_i7 |
|---|
| (A)13: Primer 1 details |
|---|
| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | CAAACTCCCGATGGACCCAA | AGATGGGGAGATCTTGGCCT | Start: 3020 |
| Tm | 59.962 | 60.029 | End: 3245 |
| Size | 20 | 20 | 226 |
| Primer 2 details |
|---|
| Sequence | CCGCCTAGCAGTGAAAGGAA | CTTGTCCGAATCGAGCGAGA | Start: 478 |
| Tm | 60.036 | 59.901 | End: 581 |
| Size | 20 | 20 | 104 |
| Primer 3 details |
|---|
| Sequence | TCACCGCCTAGCAGTGAAAG | CTTGTCCGAATCGAGCGAGA | Start: 475 |
| Tm | 60.037 | 59.901 | End: 581 |
| Size | 20 | 20 | 107 |
| Primer 4 details |
|---|
| Sequence | AACGGCTGTAGGAAGGCTTC | TGGAACAGAGTTGGAGCCAC | Start: 163 |
| Tm | 60.036 | 59.891 | End: 300 |
| Size | 20 | 20 | 138 |
| Primer 5 details |
|---|
| Sequence | GTGGCTCCAACTCTGTTCCA | TTCCTTTCACTGCTAGGCGG | Start: 281 |
| Tm | 59.891 | 60.036 | End: 497 |
| Size | 20 | 20 | 217 |
(A)27: Primer 1 details | | Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | CAAACTCCCGATGGACCCAA | AGATGGGGAGATCTTGGCCT | Start: 3020 |
| Tm | 59.962 | 60.029 | End: 3245 |
| Size | 20 | 20 | 226 |
| Primer 2 details |
|---|
| Sequence | CCGCCTAGCAGTGAAAGGAA | CTTGTCCGAATCGAGCGAGA | Start: 478 |
| Tm | 60.036 | 59.901 | End: 581 |
| Size | 20 | 20 | 104 |
| Primer 3 details |
|---|
| Sequence | TCACCGCCTAGCAGTGAAAG | CTTGTCCGAATCGAGCGAGA | Start: 475 |
| Tm | 60.037 | 59.901 | End: 581 |
| Size | 20 | 20 | 107 |
| Primer 4 details |
|---|
| Sequence | AACGGCTGTAGGAAGGCTTC | TGGAACAGAGTTGGAGCCAC | Start: 163 |
| Tm | 60.036 | 59.891 | End: 300 |
| Size | 20 | 20 | 138 |
| Primer 5 details |
|---|
| Sequence | GTGGCTCCAACTCTGTTCCA | TTCCTTTCACTGCTAGGCGG | Start: 281 |
| Tm | 59.891 | 60.036 | End: 497 |
| Size | 20 | 20 | 217 |
(T)10: Primer 1 details | | Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | CAAACTCCCGATGGACCCAA | AGATGGGGAGATCTTGGCCT | Start: 3020 |
| Tm | 59.962 | 60.029 | End: 3245 |
| Size | 20 | 20 | 226 |
| Primer 2 details |
|---|
| Sequence | CCGCCTAGCAGTGAAAGGAA | CTTGTCCGAATCGAGCGAGA | Start: 478 |
| Tm | 60.036 | 59.901 | End: 581 |
| Size | 20 | 20 | 104 |
| Primer 3 details |
|---|
| Sequence | TCACCGCCTAGCAGTGAAAG | CTTGTCCGAATCGAGCGAGA | Start: 475 |
| Tm | 60.037 | 59.901 | End: 581 |
| Size | 20 | 20 | 107 |
| Primer 4 details |
|---|
| Sequence | AACGGCTGTAGGAAGGCTTC | TGGAACAGAGTTGGAGCCAC | Start: 163 |
| Tm | 60.036 | 59.891 | End: 300 |
| Size | 20 | 20 | 138 |
| Primer 5 details |
|---|
| Sequence | GTGGCTCCAACTCTGTTCCA | TTCCTTTCACTGCTAGGCGG | Start: 281 |
| Tm | 59.891 | 60.036 | End: 497 |
| Size | 20 | 20 | 217 |
(A)11: Primer 1 details | | Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | CAAACTCCCGATGGACCCAA | AGATGGGGAGATCTTGGCCT | Start: 3020 |
| Tm | 59.962 | 60.029 | End: 3245 |
| Size | 20 | 20 | 226 |
| Primer 2 details |
|---|
| Sequence | CCGCCTAGCAGTGAAAGGAA | CTTGTCCGAATCGAGCGAGA | Start: 478 |
| Tm | 60.036 | 59.901 | End: 581 |
| Size | 20 | 20 | 104 |
| Primer 3 details |
|---|
| Sequence | TCACCGCCTAGCAGTGAAAG | CTTGTCCGAATCGAGCGAGA | Start: 475 |
| Tm | 60.037 | 59.901 | End: 581 |
| Size | 20 | 20 | 107 |
| Primer 4 details |
|---|
| Sequence | AACGGCTGTAGGAAGGCTTC | TGGAACAGAGTTGGAGCCAC | Start: 163 |
| Tm | 60.036 | 59.891 | End: 300 |
| Size | 20 | 20 | 138 |
| Primer 5 details |
|---|
| Sequence | GTGGCTCCAACTCTGTTCCA | TTCCTTTCACTGCTAGGCGG | Start: 281 |
| Tm | 59.891 | 60.036 | End: 497 |
| Size | 20 | 20 | 217 |
(TAA)5: Primer 1 details | | Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | CAAACTCCCGATGGACCCAA | AGATGGGGAGATCTTGGCCT | Start: 3020 |
| Tm | 59.962 | 60.029 | End: 3245 |
| Size | 20 | 20 | 226 |
| Primer 2 details |
|---|
| Sequence | CCGCCTAGCAGTGAAAGGAA | CTTGTCCGAATCGAGCGAGA | Start: 478 |
| Tm | 60.036 | 59.901 | End: 581 |
| Size | 20 | 20 | 104 |
| Primer 3 details |
|---|
| Sequence | TCACCGCCTAGCAGTGAAAG | CTTGTCCGAATCGAGCGAGA | Start: 475 |
| Tm | 60.037 | 59.901 | End: 581 |
| Size | 20 | 20 | 107 |
| Primer 4 details |
|---|
| Sequence | AACGGCTGTAGGAAGGCTTC | TGGAACAGAGTTGGAGCCAC | Start: 163 |
| Tm | 60.036 | 59.891 | End: 300 |
| Size | 20 | 20 | 138 |
| Primer 5 details |
|---|
| Sequence | GTGGCTCCAACTCTGTTCCA | TTCCTTTCACTGCTAGGCGG | Start: 281 |
| Tm | 59.891 | 60.036 | End: 497 |
| Size | 20 | 20 | 217 |
(GA)9: Primer 1 details | | Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | CAAACTCCCGATGGACCCAA | AGATGGGGAGATCTTGGCCT | Start: 3020 |
| Tm | 59.962 | 60.029 | End: 3245 |
| Size | 20 | 20 | 226 |
| Primer 2 details |
|---|
| Sequence | CCGCCTAGCAGTGAAAGGAA | CTTGTCCGAATCGAGCGAGA | Start: 478 |
| Tm | 60.036 | 59.901 | End: 581 |
| Size | 20 | 20 | 104 |
| Primer 3 details |
|---|
| Sequence | TCACCGCCTAGCAGTGAAAG | CTTGTCCGAATCGAGCGAGA | Start: 475 |
| Tm | 60.037 | 59.901 | End: 581 |
| Size | 20 | 20 | 107 |
| Primer 4 details |
|---|
| Sequence | AACGGCTGTAGGAAGGCTTC | TGGAACAGAGTTGGAGCCAC | Start: 163 |
| Tm | 60.036 | 59.891 | End: 300 |
| Size | 20 | 20 | 138 |
| Primer 5 details |
|---|
| Sequence | GTGGCTCCAACTCTGTTCCA | TTCCTTTCACTGCTAGGCGG | Start: 281 |
| Tm | 59.891 | 60.036 | End: 497 |
| Size | 20 | 20 | 217 |
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