ssr_primer
Primer Details for TRINITY_DN77964_c3_g5_i1 |
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| (GAAA)5: Primer 1 details |
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| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | AGAAAGAAAGAAGGAAGCAACGT | CCAGATACTTCGCATGGGGG | Start: 11 |
| Tm | 58.8 | 60.25 | End: 158 |
| Size | 23 | 20 | 148 |
| Primer 2 details |
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| Sequence | AGAAAGAAAGAAGGAAGCAACGT | CCCAGATACTTCGCATGGGG | Start: 11 |
| Tm | 58.8 | 60.25 | End: 159 |
| Size | 23 | 20 | 149 |
| Primer 3 details |
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| Sequence | AGAAAGAAAGAAGGAAGCAACGT | CGGACCCAGATACTTCGCAT | Start: 11 |
| Tm | 58.8 | 59.611 | End: 163 |
| Size | 23 | 20 | 153 |
| Primer 4 details |
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| Sequence | TGACTGCTGCATAATAAATGCCT | CGGACCCAGATACTTCGCAT | Start: 61 |
| Tm | 58.79 | 59.611 | End: 163 |
| Size | 23 | 20 | 103 |
| Primer 5 details |
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| Sequence | ATGACAAAATTGACTGCTGCAT | CCAGATACTTCGCATGGGGG | Start: 51 |
| Tm | 57.469 | 60.25 | End: 158 |
| Size | 22 | 20 | 108 |
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