ssr_primer
Primer Details for TRINITY_DN77958_c6_g2_i1 |
|---|
| (A)12: Primer 1 details |
|---|
| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | GTCGGACGTTTCCTCGTTCT | GCTCGCGAAAATGACGGATC | Start: 1716 |
| Tm | 60.04 | 60.043 | End: 1991 |
| Size | 20 | 20 | 276 |
| Primer 2 details |
|---|
| Sequence | ACCACAGTGAAAAACCCCGT | CGTTTCGATTCGGGTTGACG | Start: 1636 |
| Tm | 60.034 | 59.908 | End: 1822 |
| Size | 20 | 20 | 187 |
| Primer 3 details |
|---|
| Sequence | GTCGGACGTTTCCTCGTTCT | CGTTTCGATTCGGGTTGACG | Start: 1716 |
| Tm | 60.04 | 59.908 | End: 1822 |
| Size | 20 | 20 | 107 |
| Primer 4 details |
|---|
| Sequence | CGTCAACCCGAATCGAAACG | GCTCGCGAAAATGACGGATC | Start: 1803 |
| Tm | 59.908 | 60.043 | End: 1991 |
| Size | 20 | 20 | 189 |
| Primer 5 details |
|---|
| Sequence | GGCGACGAACTTATTTGCCC | GGCGCACTTTGTGGGTAAAG | Start: 1122 |
| Tm | 59.902 | 60.04 | End: 1375 |
| Size | 20 | 20 | 254 |
(A)15ccgcagaagtgcgcaaaaatgttaggccaacattacttaaatgggtaataacactacaaaaattaaaattggagattc(A)15: Primer 1 details | | Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | GTCGGACGTTTCCTCGTTCT | GCTCGCGAAAATGACGGATC | Start: 1716 |
| Tm | 60.04 | 60.043 | End: 1991 |
| Size | 20 | 20 | 276 |
| Primer 2 details |
|---|
| Sequence | ACCACAGTGAAAAACCCCGT | CGTTTCGATTCGGGTTGACG | Start: 1636 |
| Tm | 60.034 | 59.908 | End: 1822 |
| Size | 20 | 20 | 187 |
| Primer 3 details |
|---|
| Sequence | GTCGGACGTTTCCTCGTTCT | CGTTTCGATTCGGGTTGACG | Start: 1716 |
| Tm | 60.04 | 59.908 | End: 1822 |
| Size | 20 | 20 | 107 |
| Primer 4 details |
|---|
| Sequence | CGTCAACCCGAATCGAAACG | GCTCGCGAAAATGACGGATC | Start: 1803 |
| Tm | 59.908 | 60.043 | End: 1991 |
| Size | 20 | 20 | 189 |
| Primer 5 details |
|---|
| Sequence | GGCGACGAACTTATTTGCCC | GGCGCACTTTGTGGGTAAAG | Start: 1122 |
| Tm | 59.902 | 60.04 | End: 1375 |
| Size | 20 | 20 | 254 |
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