ssr_primer
Primer Details for TRINITY_DN77941_c1_g5_i3 |
|---|
| (T)23: Primer 1 details |
|---|
| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | GTTTTGTGCGGAGGGAGAGA | TAGGAACTGCCGATACCCCA | Start: 715 |
| Tm | 59.965 | 60.032 | End: 981 |
| Size | 20 | 20 | 267 |
| Primer 2 details |
|---|
| Sequence | GTTTTGTGCGGAGGGAGAGA | CGCATTCTAGGAACTGCCGA | Start: 715 |
| Tm | 59.965 | 60.179 | End: 988 |
| Size | 20 | 20 | 274 |
| Primer 3 details |
|---|
| Sequence | CAAGTGTACGACGGTAGGCA | TAGGAACTGCCGATACCCCA | Start: 745 |
| Tm | 59.758 | 60.032 | End: 981 |
| Size | 20 | 20 | 237 |
| Primer 4 details |
|---|
| Sequence | ACAAGTGTACGACGGTAGGC | TAGGAACTGCCGATACCCCA | Start: 744 |
| Tm | 59.758 | 60.032 | End: 981 |
| Size | 20 | 20 | 238 |
| Primer 5 details |
|---|
| Sequence | TGGGGTATCGGCAGTTCCTA | TCCTTAACCACGAGGCCATG | Start: 962 |
| Tm | 60.032 | 59.748 | End: 1128 |
| Size | 20 | 20 | 167 |
(A)10taaat(A)10: Primer 1 details | | Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | GTTTTGTGCGGAGGGAGAGA | TAGGAACTGCCGATACCCCA | Start: 715 |
| Tm | 59.965 | 60.032 | End: 981 |
| Size | 20 | 20 | 267 |
| Primer 2 details |
|---|
| Sequence | GTTTTGTGCGGAGGGAGAGA | CGCATTCTAGGAACTGCCGA | Start: 715 |
| Tm | 59.965 | 60.179 | End: 988 |
| Size | 20 | 20 | 274 |
| Primer 3 details |
|---|
| Sequence | CAAGTGTACGACGGTAGGCA | TAGGAACTGCCGATACCCCA | Start: 745 |
| Tm | 59.758 | 60.032 | End: 981 |
| Size | 20 | 20 | 237 |
| Primer 4 details |
|---|
| Sequence | ACAAGTGTACGACGGTAGGC | TAGGAACTGCCGATACCCCA | Start: 744 |
| Tm | 59.758 | 60.032 | End: 981 |
| Size | 20 | 20 | 238 |
| Primer 5 details |
|---|
| Sequence | TGGGGTATCGGCAGTTCCTA | TCCTTAACCACGAGGCCATG | Start: 962 |
| Tm | 60.032 | 59.748 | End: 1128 |
| Size | 20 | 20 | 167 |
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