ssr_primer
Primer Details for TRINITY_DN77886_c2_g1_i1 |
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| (T)10: Primer 1 details |
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| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | TACTTCCGGGCTTTGATCGG | AGGTGTGCTGTCTATGGGGT | Start: 55 |
| Tm | 59.823 | 60.548 | End: 294 |
| Size | 20 | 20 | 240 |
| Primer 2 details |
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| Sequence | CCGGGCTTTGATCGGACTAG | AGGTGTGCTGTCTATGGGGT | Start: 60 |
| Tm | 60.249 | 60.548 | End: 294 |
| Size | 20 | 20 | 235 |
| Primer 3 details |
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| Sequence | ACCTCCTCTTTTGTGTCGGG | GGGGATGCATGATCAGACCA | Start: 388 |
| Tm | 59.603 | 59.525 | End: 553 |
| Size | 20 | 20 | 166 |
| Primer 4 details |
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| Sequence | ACCTCCTCTTTTGTGTCGGG | TGGGGATGCATGATCAGACC | Start: 388 |
| Tm | 59.603 | 59.525 | End: 554 |
| Size | 20 | 20 | 167 |
| Primer 5 details |
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| Sequence | ACCCCATAGACAGCACACCT | CCCGACACAAAAGAGGAGGT | Start: 275 |
| Tm | 60.548 | 59.603 | End: 407 |
| Size | 20 | 20 | 133 |
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