ssr_primer
Primer Details for TRINITY_DN77869_c0_g2_i8 |
|---|
| (A)11: Primer 1 details |
|---|
| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | TATTGGGTCTGTTGGGTCGC | GTTCGACAGCCACACAACAC | Start: 2284 |
| Tm | 60.036 | 59.972 | End: 2464 |
| Size | 20 | 20 | 181 |
| Primer 2 details |
|---|
| Sequence | TCGCATGTCCGTTGTCATGA | GTTCGACAGCCACACAACAC | Start: 2300 |
| Tm | 60.038 | 59.972 | End: 2464 |
| Size | 20 | 20 | 165 |
| Primer 3 details |
|---|
| Sequence | TATTGGGTCTGTTGGGTCGC | GCCACACAACACTCGAGAGA | Start: 2284 |
| Tm | 60.036 | 59.968 | End: 2456 |
| Size | 20 | 20 | 173 |
| Primer 4 details |
|---|
| Sequence | TATTGGGTCTGTTGGGTCGC | AGCCACACAACACTCGAGAG | Start: 2284 |
| Tm | 60.036 | 59.967 | End: 2457 |
| Size | 20 | 20 | 174 |
| Primer 5 details |
|---|
| Sequence | TCACTCGGCAGGGGAAAATC | TCGAAGTAACCCACCCGTTG | Start: 2997 |
| Tm | 60.035 | 59.966 | End: 3257 |
| Size | 20 | 20 | 261 |
(A)13: Primer 1 details | | Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | TATTGGGTCTGTTGGGTCGC | GTTCGACAGCCACACAACAC | Start: 2284 |
| Tm | 60.036 | 59.972 | End: 2464 |
| Size | 20 | 20 | 181 |
| Primer 2 details |
|---|
| Sequence | TCGCATGTCCGTTGTCATGA | GTTCGACAGCCACACAACAC | Start: 2300 |
| Tm | 60.038 | 59.972 | End: 2464 |
| Size | 20 | 20 | 165 |
| Primer 3 details |
|---|
| Sequence | TATTGGGTCTGTTGGGTCGC | GCCACACAACACTCGAGAGA | Start: 2284 |
| Tm | 60.036 | 59.968 | End: 2456 |
| Size | 20 | 20 | 173 |
| Primer 4 details |
|---|
| Sequence | TATTGGGTCTGTTGGGTCGC | AGCCACACAACACTCGAGAG | Start: 2284 |
| Tm | 60.036 | 59.967 | End: 2457 |
| Size | 20 | 20 | 174 |
| Primer 5 details |
|---|
| Sequence | TCACTCGGCAGGGGAAAATC | TCGAAGTAACCCACCCGTTG | Start: 2997 |
| Tm | 60.035 | 59.966 | End: 3257 |
| Size | 20 | 20 | 261 |
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