ssr_primer
Primer Details for TRINITY_DN77858_c2_g2_i2 |
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| (A)12: Primer 1 details |
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| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | GGATCCCTTCCACGGAACTG | CAGGGGCCATTTGTCTGACT | Start: 82 |
| Tm | 60.108 | 59.961 | End: 204 |
| Size | 20 | 20 | 123 |
| Primer 2 details |
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| Sequence | GGATCCCTTCCACGGAACTG | AAACCAGGGGCCATTTGTCT | Start: 82 |
| Tm | 60.108 | 59.809 | End: 208 |
| Size | 20 | 20 | 127 |
| Primer 3 details |
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| Sequence | GGGCAACAATCAGCAAAGCA | CAGTGCCAAACTTGTCGCTG | Start: 642 |
| Tm | 59.966 | 60.317 | End: 874 |
| Size | 20 | 20 | 233 |
| Primer 4 details |
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| Sequence | GGGCAACAATCAGCAAAGCA | GTGCCAAACTTGTCGCTGAA | Start: 642 |
| Tm | 59.966 | 59.62 | End: 872 |
| Size | 20 | 20 | 231 |
| Primer 5 details |
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| Sequence | CAGCGACAAGTTTGGCACTG | AAGTCGCAGGAGCTGGATTC | Start: 855 |
| Tm | 60.317 | 60.108 | End: 1109 |
| Size | 20 | 20 | 255 |
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