Primer Details for TRINITY_DN77853_c4_g1_i1 | |||
|---|---|---|---|
| (A)12: Primer 1 details | |||
| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | AAGCAAGTGGAAAAGGCAAGG | TTTTTCCCCTTATGGCCTTTTTCG | Start: 0 |
| Tm | 59.586 | 60.263 | End: 99 |
| Size | 21 | 24 | 100 |
| Primer 2 details | |||
| Sequence | AAGCAAGTGGAAAAGGCAAGG | TTTTTCCCCTTATGGCCTTTTTC | Start: 0 |
| Tm | 59.586 | 57.764 | End: 99 |
| Size | 21 | 23 | 100 |
| Primer 3 details | |||
| Sequence | AAGCAAGTGGAAAAGGCAAG | TTTTTCCCCTTATGGCCTTTTTCG | Start: 0 |
| Tm | 57.103 | 60.263 | End: 99 |
| Size | 20 | 24 | 100 |
| Primer 4 details | |||
| Sequence | AAGCAAGTGGAAAAGGCAAGGA | TTTTTCCCCTTATGGCCTTTTTCG | Start: 0 |
| Tm | 60.955 | 60.263 | End: 99 |
| Size | 22 | 24 | 100 |
| Primer 5 details | |||
| Sequence | AAGCAAGTGGAAAAGGCAAGG | TTTTTCCCCTTATGGCCTTTTTCGT | Start: 0 |
| Tm | 59.586 | 61.689 | End: 99 |
| Size | 21 | 25 | 100 | (A)10g(A)12: Primer 1 details |
| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | AAGCAAGTGGAAAAGGCAAGG | TTTTTCCCCTTATGGCCTTTTTCG | Start: 0 |
| Tm | 59.586 | 60.263 | End: 99 |
| Size | 21 | 24 | 100 |
| Primer 2 details | |||
| Sequence | AAGCAAGTGGAAAAGGCAAGG | TTTTTCCCCTTATGGCCTTTTTC | Start: 0 |
| Tm | 59.586 | 57.764 | End: 99 |
| Size | 21 | 23 | 100 |
| Primer 3 details | |||
| Sequence | AAGCAAGTGGAAAAGGCAAG | TTTTTCCCCTTATGGCCTTTTTCG | Start: 0 |
| Tm | 57.103 | 60.263 | End: 99 |
| Size | 20 | 24 | 100 |
| Primer 4 details | |||
| Sequence | AAGCAAGTGGAAAAGGCAAGGA | TTTTTCCCCTTATGGCCTTTTTCG | Start: 0 |
| Tm | 60.955 | 60.263 | End: 99 |
| Size | 22 | 24 | 100 |
| Primer 5 details | |||
| Sequence | AAGCAAGTGGAAAAGGCAAGG | TTTTTCCCCTTATGGCCTTTTTCGT | Start: 0 |
| Tm | 59.586 | 61.689 | End: 99 |
| Size | 21 | 25 | 100 |
