Primer Details for TRINITY_DN77745_c1_g2_i1 |
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| (A)17: Primer 1 details |
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| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | TGAATAAAGATAGAAGGGGAATGTCC | GAGTGAGGAGGTGGAGTGGC | Start: 103 |
| Tm | 58.482 | 61.894 | End: 204 |
| Size | 26 | 20 | 102 |
| Primer 2 details |
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| Sequence | TGAATAAAGATAGAAGGGGAATGTCC | AGTGAGGAGGTGGAGTGGC | Start: 103 |
| Tm | 58.482 | 60.916 | End: 203 |
| Size | 26 | 19 | 101 |
| Primer 3 details |
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| Sequence | TGAATAAAGATAGAAGGGGAATGTCC | GAGTGAGGAGGTGGAGTGG | Start: 103 |
| Tm | 58.482 | 58.718 | End: 204 |
| Size | 26 | 19 | 102 |
| Primer 4 details |
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| Sequence | GAATAAAGATAGAAGGGGAATGTCC | GAGTGAGGAGGTGGAGTGGC | Start: 104 |
| Tm | 57.012 | 61.894 | End: 204 |
| Size | 25 | 20 | 101 |
| Primer 5 details |
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| Sequence | TTGAATAAAGATAGAAGGGGAATGTCC | GAGTGAGGAGGTGGAGTGGC | Start: 102 |
| Tm | 59.001 | 61.894 | End: 204 |
| Size | 27 | 20 | 103 |
