ssr_primer
Primer Details for TRINITY_DN77743_c0_g3_i1 |
|---|
| (AAT)5: Primer 1 details |
|---|
| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | TGCTTAAAGCGCTCAGACGA | CTGTGAATCCTGGTCCGGTT | Start: 504 |
| Tm | 60.039 | 59.675 | End: 770 |
| Size | 20 | 20 | 267 |
| Primer 2 details |
|---|
| Sequence | TGCTTAAAGCGCTCAGACGA | AACTGTGAATCCTGGTCCGG | Start: 504 |
| Tm | 60.039 | 59.675 | End: 772 |
| Size | 20 | 20 | 269 |
| Primer 3 details |
|---|
| Sequence | AACACCGGAAGAAGTCGAGG | GCAAGGGCACTTAGGTACCA | Start: 162 |
| Tm | 59.683 | 59.673 | End: 352 |
| Size | 20 | 20 | 191 |
| Primer 4 details |
|---|
| Sequence | GGCTCTGACAGTGTACCACA | TCGTCTGAGCGCTTTAAGCA | Start: 265 |
| Tm | 59.32 | 60.039 | End: 523 |
| Size | 20 | 20 | 259 |
| Primer 5 details |
|---|
| Sequence | CCAGAGACAGAAGTGCACCA | CTGTGAATCCTGGTCCGGTT | Start: 542 |
| Tm | 59.606 | 59.675 | End: 770 |
| Size | 20 | 20 | 229 |
(T)10: Primer 1 details | | Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | TGCTTAAAGCGCTCAGACGA | CTGTGAATCCTGGTCCGGTT | Start: 504 |
| Tm | 60.039 | 59.675 | End: 770 |
| Size | 20 | 20 | 267 |
| Primer 2 details |
|---|
| Sequence | TGCTTAAAGCGCTCAGACGA | AACTGTGAATCCTGGTCCGG | Start: 504 |
| Tm | 60.039 | 59.675 | End: 772 |
| Size | 20 | 20 | 269 |
| Primer 3 details |
|---|
| Sequence | AACACCGGAAGAAGTCGAGG | GCAAGGGCACTTAGGTACCA | Start: 162 |
| Tm | 59.683 | 59.673 | End: 352 |
| Size | 20 | 20 | 191 |
| Primer 4 details |
|---|
| Sequence | GGCTCTGACAGTGTACCACA | TCGTCTGAGCGCTTTAAGCA | Start: 265 |
| Tm | 59.32 | 60.039 | End: 523 |
| Size | 20 | 20 | 259 |
| Primer 5 details |
|---|
| Sequence | CCAGAGACAGAAGTGCACCA | CTGTGAATCCTGGTCCGGTT | Start: 542 |
| Tm | 59.606 | 59.675 | End: 770 |
| Size | 20 | 20 | 229 |
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