Primer Details for TRINITY_DN77742_c5_g5_i1 |
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| (A)31: Primer 1 details |
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| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | TCCCAAATGGCGTATCTCGG | TGCAGAGAAGAAAAATCAAGGAAGT | Start: 57 |
| Tm | 59.894 | 59.17 | End: 173 |
| Size | 20 | 25 | 117 |
| Primer 2 details |
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| Sequence | CCCAAATGGCGTATCTCGGT | TGCAGAGAAGAAAAATCAAGGAAGT | Start: 58 |
| Tm | 60.179 | 59.17 | End: 173 |
| Size | 20 | 25 | 116 |
| Primer 3 details |
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| Sequence | TCCCAAATGGCGTATCTCGG | TGCAGAGAAGAAAAATCAAGGAAGTT | Start: 57 |
| Tm | 59.894 | 59.678 | End: 173 |
| Size | 20 | 26 | 117 |
| Primer 4 details |
|---|
| Sequence | TCCCAAATGGCGTATCTCGG | TTGCAGAGAAGAAAAATCAAGGAAGT | Start: 57 |
| Tm | 59.894 | 59.678 | End: 174 |
| Size | 20 | 26 | 118 |
| Primer 5 details |
|---|
| Sequence | TCCCAAATGGCGTATCTCGG | TGCAGAGAAGAAAAATCAAGGAAG | Start: 57 |
| Tm | 59.894 | 57.677 | End: 173 |
| Size | 20 | 24 | 117 |
