ssr_primer
Primer Details for TRINITY_DN77735_c4_g7_i1 |
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| (A)16: Primer 1 details |
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| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | AGGCCTTTCGGAAAGAACGT | TGGAAAATGAACGTTCTTTCCGA | Start: 123 |
| Tm | 59.892 | 59.122 | End: 248 |
| Size | 20 | 23 | 126 |
| Primer 2 details |
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| Sequence | TTAGCTCCGAGTCGAGGGAT | TGGAAAATGAACGTTCTTTCCGA | Start: 79 |
| Tm | 59.817 | 59.122 | End: 248 |
| Size | 20 | 23 | 170 |
| Primer 3 details |
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| Sequence | CTCCGAGTCGAGGGATCGTA | TGGAAAATGAACGTTCTTTCCGA | Start: 83 |
| Tm | 60.25 | 59.122 | End: 248 |
| Size | 20 | 23 | 166 |
| Primer 4 details |
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| Sequence | TAGCTCCGAGTCGAGGGATC | TGGAAAATGAACGTTCTTTCCGA | Start: 80 |
| Tm | 60.25 | 59.122 | End: 248 |
| Size | 20 | 23 | 169 |
| Primer 5 details |
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| Sequence | CTGTTAGCTCCGAGTCGAGG | TGGAAAATGAACGTTCTTTCCGA | Start: 76 |
| Tm | 59.621 | 59.122 | End: 248 |
| Size | 20 | 23 | 173 |
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