Primer Details for TRINITY_DN77734_c7_g1_i1 |
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| (T)15: Primer 1 details |
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| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | TTGAAATCACACCTAAACTACAATACT | ACTGACAGCTTGTAGAAGGCA | Start: 0 |
| Tm | 57.213 | 59.306 | End: 110 |
| Size | 27 | 21 | 111 |
| Primer 2 details |
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| Sequence | TTGAAATCACACCTAAACTACAATACT | ACTGACAGCTTGTAGAAGGCAA | Start: 0 |
| Tm | 57.213 | 59.895 | End: 110 |
| Size | 27 | 22 | 111 |
| Primer 3 details |
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| Sequence | TTGAAATCACACCTAAACTACAATACT | CTGACAGCTTGTAGAAGGCA | Start: 0 |
| Tm | 57.213 | 57.538 | End: 109 |
| Size | 27 | 20 | 110 |
| Primer 4 details |
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| Sequence | TTGAAATCACACCTAAACTACAATACT | ACTGACAGCTTGTAGAAGGC | Start: 0 |
| Tm | 57.213 | 57.536 | End: 110 |
| Size | 27 | 20 | 111 |
| Primer 5 details |
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| Sequence | TTGAAATCACACCTAAACTACAATACT | CTGACAGCTTGTAGAAGGCAA | Start: 0 |
| Tm | 57.213 | 58.229 | End: 109 |
| Size | 27 | 21 | 110 |
