ssr_primer
Primer Details for TRINITY_DN77732_c5_g7_i1 |
|---|
| (T)12: Primer 1 details |
|---|
| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | TTGTGGGTCGCTAAACCTCC | TTTTTATTGCCACACCGCGG | Start: 323 |
| Tm | 59.964 | 60.039 | End: 433 |
| Size | 20 | 20 | 111 |
| Primer 2 details |
|---|
| Sequence | GTGGGTCGCTAAACCTCCTC | TTTTTATTGCCACACCGCGG | Start: 325 |
| Tm | 60.108 | 60.039 | End: 433 |
| Size | 20 | 20 | 109 |
| Primer 3 details |
|---|
| Sequence | TTGTGGGTCGCTAAACCTCC | CCGCTTCGGTCTTGAGGTTA | Start: 323 |
| Tm | 59.964 | 59.754 | End: 487 |
| Size | 20 | 20 | 165 |
| Primer 4 details |
|---|
| Sequence | TTGTGGGTCGCTAAACCTCC | CGCCGTCGCTCTTTCATAGA | Start: 323 |
| Tm | 59.964 | 60.248 | End: 461 |
| Size | 20 | 20 | 139 |
| Primer 5 details |
|---|
| Sequence | TTGTGGGTCGCTAAACCTCC | ATTTGCACTGACAGACCGCT | Start: 323 |
| Tm | 59.964 | 60.25 | End: 502 |
| Size | 20 | 20 | 180 |
(T)18: Primer 1 details | | Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | TTGTGGGTCGCTAAACCTCC | TTTTTATTGCCACACCGCGG | Start: 323 |
| Tm | 59.964 | 60.039 | End: 433 |
| Size | 20 | 20 | 111 |
| Primer 2 details |
|---|
| Sequence | GTGGGTCGCTAAACCTCCTC | TTTTTATTGCCACACCGCGG | Start: 325 |
| Tm | 60.108 | 60.039 | End: 433 |
| Size | 20 | 20 | 109 |
| Primer 3 details |
|---|
| Sequence | TTGTGGGTCGCTAAACCTCC | CCGCTTCGGTCTTGAGGTTA | Start: 323 |
| Tm | 59.964 | 59.754 | End: 487 |
| Size | 20 | 20 | 165 |
| Primer 4 details |
|---|
| Sequence | TTGTGGGTCGCTAAACCTCC | CGCCGTCGCTCTTTCATAGA | Start: 323 |
| Tm | 59.964 | 60.248 | End: 461 |
| Size | 20 | 20 | 139 |
| Primer 5 details |
|---|
| Sequence | TTGTGGGTCGCTAAACCTCC | ATTTGCACTGACAGACCGCT | Start: 323 |
| Tm | 59.964 | 60.25 | End: 502 |
| Size | 20 | 20 | 180 |
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