ssr_primer
Primer Details for TRINITY_DN77732_c5_g5_i1 |
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| (T)24: Primer 1 details |
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| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | CGCGAAAATCCCCATTCCAC | GTCGGGGGAAATTTGGCAAC | Start: 428 |
| Tm | 59.899 | 60.038 | End: 631 |
| Size | 20 | 20 | 204 |
| Primer 2 details |
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| Sequence | CACCGCGAAAATCCCCATTC | GTCGGGGGAAATTTGGCAAC | Start: 425 |
| Tm | 59.899 | 60.038 | End: 631 |
| Size | 20 | 20 | 207 |
| Primer 3 details |
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| Sequence | TATTTTCGCGAGAGTCCCGG | GTCGGGGGAAATTTGGCAAC | Start: 393 |
| Tm | 59.898 | 60.038 | End: 631 |
| Size | 20 | 20 | 239 |
| Primer 4 details |
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| Sequence | GCGAGAGTCCCGGAAAATCA | GTCGGGGGAAATTTGGCAAC | Start: 400 |
| Tm | 60.108 | 60.038 | End: 631 |
| Size | 20 | 20 | 232 |
| Primer 5 details |
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| Sequence | GGGAAGACAACGTCGCCATA | GTCGGGGGAAATTTGGCAAC | Start: 375 |
| Tm | 60.109 | 60.038 | End: 631 |
| Size | 20 | 20 | 257 |
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