ssr_primer
Primer Details for TRINITY_DN77732_c5_g3_i1 |
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| (A)12ggatagtaattttagaattgcaagactataggatacatttttttcaacgcatttgcatttagcatggagtaatatatatagaaaattaaaaatgaaat(A)12: Primer 1 details |
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| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | GTATCCAAGTCGAGAGCCGG | GAGGGAGGAGGAAGGCTGTA | Start: 177 |
| Tm | 59.969 | 60.032 | End: 303 |
| Size | 20 | 20 | 127 |
| Primer 2 details |
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| Sequence | GTATCCAAGTCGAGAGCCGG | GAAGTGTGTGGGTGGAGAGG | Start: 177 |
| Tm | 59.969 | 59.965 | End: 319 |
| Size | 20 | 20 | 143 |
| Primer 3 details |
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| Sequence | CTTGCCCACATCGTTCCTCT | CCGGCTCTCGACTTGGATAC | Start: 3 |
| Tm | 60.036 | 59.969 | End: 196 |
| Size | 20 | 20 | 194 |
| Primer 4 details |
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| Sequence | TCTACCTTCGGACGGCAGTA | GAGGGAGGAGGAAGGCTGTA | Start: 160 |
| Tm | 60.035 | 60.032 | End: 303 |
| Size | 20 | 20 | 144 |
| Primer 5 details |
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| Sequence | TCTACCTTCGGACGGCAGTA | GAAGTGTGTGGGTGGAGAGG | Start: 160 |
| Tm | 60.035 | 59.965 | End: 319 |
| Size | 20 | 20 | 160 |
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