ssr_primer
Primer Details for TRINITY_DN77732_c3_g1_i1 |
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| (G)10: Primer 1 details |
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| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | TTTTACCCCACCAGCAAGGA | AACTACTGAAAAGCCAGCGT | Start: 1 |
| Tm | 59.146 | 57.744 | End: 156 |
| Size | 20 | 20 | 156 |
| Primer 2 details |
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| Sequence | TTTTACCCCACCAGCAAGGA | AACAGCAGCTTTCAAGAGGT | Start: 1 |
| Tm | 59.146 | 57.645 | End: 124 |
| Size | 20 | 20 | 124 |
| Primer 3 details |
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| Sequence | TTTTACCCCACCAGCAAGGA | CAAACAGCAGCTTTCAAGAGGT | Start: 1 |
| Tm | 59.146 | 59.64 | End: 126 |
| Size | 20 | 22 | 126 |
| Primer 4 details |
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| Sequence | TTTTACCCCACCAGCAAGGA | ACAAACAGCAGCTTTCAAGAGG | Start: 1 |
| Tm | 59.146 | 59.64 | End: 127 |
| Size | 20 | 22 | 127 |
| Primer 5 details |
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| Sequence | GGCAGACCCCTATTCCTTCC | GCGATTGATATTGCTCGTGAGT | Start: 64 |
| Tm | 59.525 | 59.203 | End: 211 |
| Size | 20 | 22 | 148 |
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