Primer Details for TRINITY_DN77728_c3_g2_i1 |
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| (T)11gatttcttacgtttcctttcattttctttttcattttcttatttccttattttactttttttactattacttttatttttcttttc(T)15c(T)15ctttcttttgtttttcttcttgtttgctttttcttcgttttc(T)11: Primer 1 details |
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| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | ACGTTTCCTTTCATTTTCTTTTTCAT | ACGAAGAAAAAGCAAACAAGAAGA | Start: 29 |
| Tm | 57.283 | 58.05 | End: 175 |
| Size | 26 | 24 | 147 |
| Primer 2 details |
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| Sequence | ACGTTTCCTTTCATTTTCTTTTTCAT | ACGAAGAAAAAGCAAACAAGAAGAA | Start: 29 |
| Tm | 57.283 | 58.613 | End: 175 |
| Size | 26 | 25 | 147 |
| Primer 3 details |
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| Sequence | ACGTTTCCTTTCATTTTCTTTTTCAT | AACGAAGAAAAAGCAAACAAGAAGA | Start: 29 |
| Tm | 57.283 | 58.613 | End: 176 |
| Size | 26 | 25 | 148 |
| Primer 4 details |
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| Sequence | ACGTTTCCTTTCATTTTCTTTTTCATT | ACGAAGAAAAAGCAAACAAGAAGA | Start: 29 |
| Tm | 57.838 | 58.05 | End: 175 |
| Size | 27 | 24 | 147 |
| Primer 5 details |
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| Sequence | ACGTTTCCTTTCATTTTCTTTTTCAT | AACGAAGAAAAAGCAAACAAGAAG | Start: 29 |
| Tm | 57.283 | 57.368 | End: 176 |
| Size | 26 | 24 | 148 |
