ssr_primer
Primer Details for TRINITY_DN77726_c4_g1_i1 |
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| (T)10: Primer 1 details |
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| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | TTTGCAGCGTCCGGAAAAAG | ACACCCAACTTTTCGCTAAACA | Start: 52 |
| Tm | 59.97 | 58.982 | End: 268 |
| Size | 20 | 22 | 217 |
| Primer 2 details |
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| Sequence | GAATTTGCAGCGTCCGGAAA | ACACCCAACTTTTCGCTAAACA | Start: 49 |
| Tm | 59.76 | 58.982 | End: 268 |
| Size | 20 | 22 | 220 |
| Primer 3 details |
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| Sequence | TTTGCAGCGTCCGGAAAAAG | AACACCCAACTTTTCGCTAAACA | Start: 52 |
| Tm | 59.97 | 59.556 | End: 269 |
| Size | 20 | 23 | 218 |
| Primer 4 details |
|---|
| Sequence | TTTGCAGCGTCCGGAAAAAG | AAAAACACCCAACTTTTCGCT | Start: 52 |
| Tm | 59.97 | 57.397 | End: 272 |
| Size | 20 | 21 | 221 |
| Primer 5 details |
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| Sequence | TTTGCAGCGTCCGGAAAAAG | ACACCCAACTTTTCGCTAAACAT | Start: 52 |
| Tm | 59.97 | 59.365 | End: 268 |
| Size | 20 | 23 | 217 |
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