ssr_primer
Primer Details for TRINITY_DN77722_c3_g2_i1 |
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| (T)10: Primer 1 details |
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| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | GCTACCGCCACCCATGAAAA | TTGCCTCTGAATTTCCGGCT | Start: 80 |
| Tm | 60.963 | 59.962 | End: 293 |
| Size | 20 | 20 | 214 |
| Primer 2 details |
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| Sequence | GCTACCGCCACCCATGAAAA | GTTGCCTCTGAATTTCCGGC | Start: 80 |
| Tm | 60.963 | 59.829 | End: 294 |
| Size | 20 | 20 | 215 |
| Primer 3 details |
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| Sequence | GCCGGAAATTCAGAGGCAAC | CAACAAAAGGCACGGTTTCCA | Start: 275 |
| Tm | 59.829 | 60.135 | End: 461 |
| Size | 20 | 21 | 187 |
| Primer 4 details |
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| Sequence | GCCGGAAATTCAGAGGCAAC | TCAACAAAAGGCACGGTTTCC | Start: 275 |
| Tm | 59.829 | 59.865 | End: 462 |
| Size | 20 | 21 | 188 |
| Primer 5 details |
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| Sequence | GCCGGAAATTCAGAGGCAAC | AACAAAAGGCACGGTTTCCA | Start: 275 |
| Tm | 59.829 | 58.813 | End: 460 |
| Size | 20 | 20 | 186 |
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