Primer Details for TRINITY_DN77718_c7_g1_i1 |
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| (G)13t(G)10a(G)12c(G)13agggggggggcggggggggtggggga(G)17: Primer 1 details |
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| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | CAAACCACAGAAAGAATACATAATCTC | TTTTCCCCAAATCTCCCCCA | Start: 0 |
| Tm | 57.006 | 58.828 | End: 99 |
| Size | 27 | 20 | 100 |
| Primer 2 details |
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| Sequence | CAAACCACAGAAAGAATACATAATCTC | TTTTCCCCAAATCTCCCCCAC | Start: 0 |
| Tm | 57.006 | 60.203 | End: 99 |
| Size | 27 | 21 | 100 |
| Primer 3 details |
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| Sequence | AAACCACAGAAAGAATACATAATCTCT | TTTTTCCCCAAATCTCCCCCA | Start: 1 |
| Tm | 57.034 | 59.49 | End: 100 |
| Size | 27 | 21 | 100 |
| Primer 4 details |
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| Sequence | CAAACCACAGAAAGAATACATAATCTC | TTTTTCCCCAAATCTCCCCCA | Start: 0 |
| Tm | 57.006 | 59.49 | End: 100 |
| Size | 27 | 21 | 101 |
| Primer 5 details |
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| Sequence | AAACCACAGAAAGAATACATAATCTCT | TTTTTCCCCAAATCTCCCCC | Start: 1 |
| Tm | 57.034 | 57.664 | End: 100 |
| Size | 27 | 20 | 100 |
