ssr_primer
Primer Details for TRINITY_DN77718_c2_g1_i1 |
|---|
| (A)20: Primer 1 details |
|---|
| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | TTGGACGGCCCCTGATTTTT | ACAGTCACCAGAAACGGCAT | Start: 253 |
| Tm | 60.179 | 59.891 | End: 373 |
| Size | 20 | 20 | 121 |
| Primer 2 details |
|---|
| Sequence | GGCTGTCTGGCTCAGAGTTT | TTTGACTCCGCACGCTAGAA | Start: 297 |
| Tm | 59.964 | 59.684 | End: 408 |
| Size | 20 | 20 | 112 |
| Primer 3 details |
|---|
| Sequence | GGCTGTCTGGCTCAGAGTTT | TTTTGACTCCGCACGCTAGA | Start: 297 |
| Tm | 59.964 | 59.684 | End: 409 |
| Size | 20 | 20 | 113 |
| Primer 4 details |
|---|
| Sequence | GGCTGTCTGGCTCAGAGTTT | AACGGCATCTAAAGGTGGCT | Start: 297 |
| Tm | 59.964 | 59.672 | End: 494 |
| Size | 20 | 20 | 198 |
| Primer 5 details |
|---|
| Sequence | GGCTGTCTGGCTCAGAGTTT | TTGACTCCGCACGCTAGAAC | Start: 297 |
| Tm | 59.964 | 60.388 | End: 407 |
| Size | 20 | 20 | 111 |
(G)12: Primer 1 details | | Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | TTGGACGGCCCCTGATTTTT | ACAGTCACCAGAAACGGCAT | Start: 253 |
| Tm | 60.179 | 59.891 | End: 373 |
| Size | 20 | 20 | 121 |
| Primer 2 details |
|---|
| Sequence | GGCTGTCTGGCTCAGAGTTT | TTTGACTCCGCACGCTAGAA | Start: 297 |
| Tm | 59.964 | 59.684 | End: 408 |
| Size | 20 | 20 | 112 |
| Primer 3 details |
|---|
| Sequence | GGCTGTCTGGCTCAGAGTTT | TTTTGACTCCGCACGCTAGA | Start: 297 |
| Tm | 59.964 | 59.684 | End: 409 |
| Size | 20 | 20 | 113 |
| Primer 4 details |
|---|
| Sequence | GGCTGTCTGGCTCAGAGTTT | AACGGCATCTAAAGGTGGCT | Start: 297 |
| Tm | 59.964 | 59.672 | End: 494 |
| Size | 20 | 20 | 198 |
| Primer 5 details |
|---|
| Sequence | GGCTGTCTGGCTCAGAGTTT | TTGACTCCGCACGCTAGAAC | Start: 297 |
| Tm | 59.964 | 60.388 | End: 407 |
| Size | 20 | 20 | 111 |
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