ssr_primer
Primer Details for TRINITY_DN77689_c2_g4_i2 |
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| (T)14: Primer 1 details |
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| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | ACCACCTAGGCCAATTCAGC | ACCGGACTGTAGATGACGGA | Start: 1382 |
| Tm | 60.034 | 60.035 | End: 1502 |
| Size | 20 | 20 | 121 |
| Primer 2 details |
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| Sequence | CTTCTGCGGTTAGGTGACGT | GCTGAATTGGCCTAGGTGGT | Start: 1201 |
| Tm | 60.039 | 60.034 | End: 1401 |
| Size | 20 | 20 | 201 |
| Primer 3 details |
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| Sequence | TACGGGCCGTCAGACAAAAA | ATGCAACGCCCACACTTTTC | Start: 320 |
| Tm | 59.894 | 59.968 | End: 431 |
| Size | 20 | 20 | 112 |
| Primer 4 details |
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| Sequence | CAACGGATACCACCTAGGCC | ACCGGACTGTAGATGACGGA | Start: 1374 |
| Tm | 59.894 | 60.035 | End: 1502 |
| Size | 20 | 20 | 129 |
| Primer 5 details |
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| Sequence | TTCGAAATGGACAGAGGGGC | TTTTTGTCTGACGGCCCGTA | Start: 74 |
| Tm | 60.035 | 59.894 | End: 339 |
| Size | 20 | 20 | 266 |
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