ssr_primer
Primer Details for TRINITY_DN77610_c4_g1_i3 |
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| (A)10: Primer 1 details |
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| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | AGTGCGAGATTGGACCAGTG | GGAAGGGGCTTGAAACCACT | Start: 181 |
| Tm | 60.037 | 60.179 | End: 433 |
| Size | 20 | 20 | 253 |
| Primer 2 details |
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| Sequence | AGTGCGAGATTGGACCAGTG | TTGTTATCTGGCCGGGAAGG | Start: 181 |
| Tm | 60.037 | 59.745 | End: 447 |
| Size | 20 | 20 | 267 |
| Primer 3 details |
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| Sequence | GCGAGATTGGACCAGTGGAT | GGAAGGGGCTTGAAACCACT | Start: 184 |
| Tm | 59.821 | 60.179 | End: 433 |
| Size | 20 | 20 | 250 |
| Primer 4 details |
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| Sequence | AGTGGTTTCAAGCCCCTTCC | GACGTATCGGGGTGGTTTCA | Start: 414 |
| Tm | 60.179 | 59.753 | End: 584 |
| Size | 20 | 20 | 171 |
| Primer 5 details |
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| Sequence | AGTGGTTTCAAGCCCCTTCC | TCGGGGTGGTTTCAACATCC | Start: 414 |
| Tm | 60.179 | 60.251 | End: 578 |
| Size | 20 | 20 | 165 |
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