ssr_primer
Primer Details for TRINITY_DN77583_c0_g1_i1 |
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| (A)13: Primer 1 details |
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| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | CCGGCCTTTTGAATTTGCGA | AGTCACAAACGGCCAATTGC | Start: 27 |
| Tm | 60.039 | 59.968 | End: 275 |
| Size | 20 | 20 | 249 |
| Primer 2 details |
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| Sequence | CCGGCCTTTTGAATTTGCGA | GCCAATTGCGCTTCAGAGAC | Start: 27 |
| Tm | 60.039 | 60.179 | End: 264 |
| Size | 20 | 20 | 238 |
| Primer 3 details |
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| Sequence | TCCCGGCCTTTTGAATTTGC | AGTCACAAACGGCCAATTGC | Start: 25 |
| Tm | 59.68 | 59.968 | End: 275 |
| Size | 20 | 20 | 251 |
| Primer 4 details |
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| Sequence | GGCTAATGGATGCTCCGGAT | AGTCACAAACGGCCAATTGC | Start: 81 |
| Tm | 59.674 | 59.968 | End: 275 |
| Size | 20 | 20 | 195 |
| Primer 5 details |
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| Sequence | GGCTAATGGATGCTCCGGAT | CTGGTTTACGGCCCTTCGAT | Start: 81 |
| Tm | 59.674 | 60.108 | End: 332 |
| Size | 20 | 20 | 252 |
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