Primer Details for TRINITY_DN77579_c1_g1_i1 |
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| (T)14(G)11: Primer 1 details |
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| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | GGGGGATATTTACAATTTTTCCAGT | ACACCTAACCACCACACTGC | Start: 82 |
| Tm | 57.568 | 60.179 | End: 194 |
| Size | 25 | 20 | 113 |
| Primer 2 details |
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| Sequence | GGGGGATATTTACAATTTTTCCAGT | CGATCTGCCACACCTAACCA | Start: 82 |
| Tm | 57.568 | 59.751 | End: 203 |
| Size | 25 | 20 | 122 |
| Primer 3 details |
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| Sequence | GGGGGATATTTACAATTTTTCCAGT | ACGATCTGCCACACCTAACC | Start: 82 |
| Tm | 57.568 | 59.751 | End: 204 |
| Size | 25 | 20 | 123 |
| Primer 4 details |
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| Sequence | GGGGGATATTTACAATTTTTCCAGT | ACACTGCCGTGCTAAGGAAG | Start: 82 |
| Tm | 57.568 | 60.321 | End: 181 |
| Size | 25 | 20 | 100 |
| Primer 5 details |
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| Sequence | GGGGGATATTTACAATTTTTCCAGT | ATCTGCCACACCTAACCACC | Start: 82 |
| Tm | 57.568 | 59.672 | End: 201 |
| Size | 25 | 20 | 120 |
