ssr_primer
Primer Details for TRINITY_DN77535_c1_g1_i1 |
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| (T)10: Primer 1 details |
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| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | TGCAAACGATCTGCAAAGCA | TTCTTCACCACACCGATGCC | Start: 753 |
| Tm | 59.333 | 60.605 | End: 859 |
| Size | 20 | 20 | 107 |
| Primer 2 details |
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| Sequence | CCGCGCGGAAATATTTTCACA | CCATCAACGCTGCAAGACTG | Start: 614 |
| Tm | 60.202 | 59.833 | End: 790 |
| Size | 21 | 20 | 177 |
| Primer 3 details |
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| Sequence | CCGCGCGGAAATATTTTCACA | TCCATTCGATTCAGCGCTGA | Start: 614 |
| Tm | 60.202 | 59.824 | End: 825 |
| Size | 21 | 20 | 212 |
| Primer 4 details |
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| Sequence | GGGGCAAAATTTTAAGGCGC | AATTGGGGCTCGCACTTGAT | Start: 3 |
| Tm | 58.917 | 60.324 | End: 171 |
| Size | 20 | 20 | 169 |
| Primer 5 details |
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| Sequence | CCGCGCGGAAATATTTTCACA | TTCCCATCAACGCTGCAAGA | Start: 614 |
| Tm | 60.202 | 60.251 | End: 793 |
| Size | 21 | 20 | 180 |
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