ssr_primer
Primer Details for TRINITY_DN77528_c0_g1_i1 |
|---|
| (A)12: Primer 1 details |
|---|
| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | TTTTTGCCCCTTCCGACCTT | TCGGGGGAACTTTCAAGCAA | Start: 867 |
| Tm | 60.106 | 59.816 | End: 967 |
| Size | 20 | 20 | 101 |
| Primer 2 details |
|---|
| Sequence | TTTTTGCCCCTTCCGACCTT | CTCGGGGGAACTTTCAAGCA | Start: 867 |
| Tm | 60.106 | 60.251 | End: 968 |
| Size | 20 | 20 | 102 |
| Primer 3 details |
|---|
| Sequence | TTTTTGCCCCTTCCGACCTT | ACTCGGGGGAACTTTCAAGC | Start: 867 |
| Tm | 60.106 | 60.251 | End: 969 |
| Size | 20 | 20 | 103 |
| Primer 4 details |
|---|
| Sequence | TGACTTTTTGCCCCTTCCGA | TCGGGGGAACTTTCAAGCAA | Start: 863 |
| Tm | 59.816 | 59.816 | End: 967 |
| Size | 20 | 20 | 105 |
| Primer 5 details |
|---|
| Sequence | ACGCATCACTGTCACAGGTT | AGCCCGTAGAGGTACTTGCT | Start: 476 |
| Tm | 59.893 | 60.324 | End: 578 |
| Size | 20 | 20 | 103 |
(A)10: Primer 1 details | | Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | TTTTTGCCCCTTCCGACCTT | TCGGGGGAACTTTCAAGCAA | Start: 867 |
| Tm | 60.106 | 59.816 | End: 967 |
| Size | 20 | 20 | 101 |
| Primer 2 details |
|---|
| Sequence | TTTTTGCCCCTTCCGACCTT | CTCGGGGGAACTTTCAAGCA | Start: 867 |
| Tm | 60.106 | 60.251 | End: 968 |
| Size | 20 | 20 | 102 |
| Primer 3 details |
|---|
| Sequence | TTTTTGCCCCTTCCGACCTT | ACTCGGGGGAACTTTCAAGC | Start: 867 |
| Tm | 60.106 | 60.251 | End: 969 |
| Size | 20 | 20 | 103 |
| Primer 4 details |
|---|
| Sequence | TGACTTTTTGCCCCTTCCGA | TCGGGGGAACTTTCAAGCAA | Start: 863 |
| Tm | 59.816 | 59.816 | End: 967 |
| Size | 20 | 20 | 105 |
| Primer 5 details |
|---|
| Sequence | ACGCATCACTGTCACAGGTT | AGCCCGTAGAGGTACTTGCT | Start: 476 |
| Tm | 59.893 | 60.324 | End: 578 |
| Size | 20 | 20 | 103 |
(A)10(ATAAATAAATAAATAAATATTTTATTGAAAAGTGTACCTTGTGTTATTGTTTTCTATAATCTAAATCGTT)(AAAT)4: Primer 1 details | | Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | TTTTTGCCCCTTCCGACCTT | TCGGGGGAACTTTCAAGCAA | Start: 867 |
| Tm | 60.106 | 59.816 | End: 967 |
| Size | 20 | 20 | 101 |
| Primer 2 details |
|---|
| Sequence | TTTTTGCCCCTTCCGACCTT | CTCGGGGGAACTTTCAAGCA | Start: 867 |
| Tm | 60.106 | 60.251 | End: 968 |
| Size | 20 | 20 | 102 |
| Primer 3 details |
|---|
| Sequence | TTTTTGCCCCTTCCGACCTT | ACTCGGGGGAACTTTCAAGC | Start: 867 |
| Tm | 60.106 | 60.251 | End: 969 |
| Size | 20 | 20 | 103 |
| Primer 4 details |
|---|
| Sequence | TGACTTTTTGCCCCTTCCGA | TCGGGGGAACTTTCAAGCAA | Start: 863 |
| Tm | 59.816 | 59.816 | End: 967 |
| Size | 20 | 20 | 105 |
| Primer 5 details |
|---|
| Sequence | ACGCATCACTGTCACAGGTT | AGCCCGTAGAGGTACTTGCT | Start: 476 |
| Tm | 59.893 | 60.324 | End: 578 |
| Size | 20 | 20 | 103 |
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