ssr_primer
Primer Details for TRINITY_DN77520_c3_g1_i1 |
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| (A)26: Primer 1 details |
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| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | AATCGCCTAACTTCTCCCCG | AGGGATGGAGATACGCCCTT | Start: 18 |
| Tm | 59.536 | 60.105 | End: 275 |
| Size | 20 | 20 | 258 |
| Primer 2 details |
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| Sequence | CTAACTTCTCCCCGCGTCTT | AGGGATGGAGATACGCCCTT | Start: 24 |
| Tm | 59.469 | 60.105 | End: 275 |
| Size | 20 | 20 | 252 |
| Primer 3 details |
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| Sequence | CTAACTTCTCCCCGCGTCTT | AATTCATGCACCTTGGGGCT | Start: 24 |
| Tm | 59.469 | 60.252 | End: 300 |
| Size | 20 | 20 | 277 |
| Primer 4 details |
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| Sequence | AATCGCCTAACTTCTCCCCG | ATGCAGGGATGGAGATACGC | Start: 18 |
| Tm | 59.536 | 59.677 | End: 279 |
| Size | 20 | 20 | 262 |
| Primer 5 details |
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| Sequence | CTTCTCCCCGCGTCTTATCA | AGGGATGGAGATACGCCCTT | Start: 28 |
| Tm | 59.255 | 60.105 | End: 275 |
| Size | 20 | 20 | 248 |
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