ssr_primer
Primer Details for TRINITY_DN77470_c0_g1_i3 |
|---|
| (A)13: Primer 1 details |
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| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | CGGAGGAGGAAGTTGATCGG | ACACGAGTTCTGCAAGGAGG | Start: 365 |
| Tm | 59.897 | 59.966 | End: 544 |
| Size | 20 | 20 | 180 |
| Primer 2 details |
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| Sequence | CGGAGGAGGAAGTTGATCGG | AGCTGTGCGACCACTACTTC | Start: 365 |
| Tm | 59.897 | 60.038 | End: 515 |
| Size | 20 | 20 | 151 |
| Primer 3 details |
|---|
| Sequence | CGGAGGAGGAAGTTGATCGG | GAAGCTGTGCGACCACTACT | Start: 365 |
| Tm | 59.897 | 60.038 | End: 517 |
| Size | 20 | 20 | 153 |
| Primer 4 details |
|---|
| Sequence | CTCCGATTTGAAGAGCCGGT | ACACGAGTTCTGCAAGGAGG | Start: 283 |
| Tm | 60.108 | 59.966 | End: 544 |
| Size | 20 | 20 | 262 |
| Primer 5 details |
|---|
| Sequence | TCCGATTTGAAGAGCCGGTC | ACACGAGTTCTGCAAGGAGG | Start: 284 |
| Tm | 60.108 | 59.966 | End: 544 |
| Size | 20 | 20 | 261 |
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