ssr_primer
Primer Details for TRINITY_DN77458_c1_g6_i1 |
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| (A)14: Primer 1 details |
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| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | GAATTTTGACGCCGAGGACG | AACGAATCGTCACCCTCCAC | Start: 1136 |
| Tm | 59.905 | 60.038 | End: 1409 |
| Size | 20 | 20 | 274 |
| Primer 2 details |
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| Sequence | GAGGGCGAAGATGAAGAGGG | AACGAATCGTCACCCTCCAC | Start: 1158 |
| Tm | 59.894 | 60.038 | End: 1409 |
| Size | 20 | 20 | 252 |
| Primer 3 details |
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| Sequence | AGATTGGGTGGAAACCGGTC | CCCTCTTCATCTTCGCCCTC | Start: 1057 |
| Tm | 59.962 | 59.894 | End: 1177 |
| Size | 20 | 20 | 121 |
| Primer 4 details |
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| Sequence | ATTTTGACGCCGAGGACGAT | AACGAATCGTCACCCTCCAC | Start: 1138 |
| Tm | 60.108 | 60.038 | End: 1409 |
| Size | 20 | 20 | 272 |
| Primer 5 details |
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| Sequence | AGATTGGGTGGAAACCGGTC | ATCGTCCTCGGCGTCAAAAT | Start: 1057 |
| Tm | 59.962 | 60.108 | End: 1157 |
| Size | 20 | 20 | 101 |
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