ssr_primer
Primer Details for TRINITY_DN77444_c1_g1_i3 |
|---|
| (A)15: Primer 1 details |
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| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | TCGAATGCCTGGTGTAGCTG | GCGTTGTTGCGATATCCACC | Start: 1878 |
| Tm | 60.108 | 59.972 | End: 1999 |
| Size | 20 | 20 | 122 |
| Primer 2 details |
|---|
| Sequence | TCGAATGCCTGGTGTAGCTG | TGCGATATCCACCGTAGTGC | Start: 1878 |
| Tm | 60.108 | 59.968 | End: 1992 |
| Size | 20 | 20 | 115 |
| Primer 3 details |
|---|
| Sequence | AAAAGCTCCTGGACCCGATG | CGTGACGTGATTCTCCTCCC | Start: 1023 |
| Tm | 60.035 | 60.179 | End: 1288 |
| Size | 20 | 20 | 266 |
| Primer 4 details |
|---|
| Sequence | AGGCAGATCCACCATTGACG | GAGGACATGCAGCCATCAGT | Start: 1322 |
| Tm | 60.108 | 60.107 | End: 1421 |
| Size | 20 | 20 | 100 |
| Primer 5 details |
|---|
| Sequence | AGGCAGATCCACCATTGACG | GTCTGAGGACATGCAGCCAT | Start: 1322 |
| Tm | 60.108 | 60.107 | End: 1425 |
| Size | 20 | 20 | 104 |
(A)28: Primer 1 details | | Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | TCGAATGCCTGGTGTAGCTG | GCGTTGTTGCGATATCCACC | Start: 1878 |
| Tm | 60.108 | 59.972 | End: 1999 |
| Size | 20 | 20 | 122 |
| Primer 2 details |
|---|
| Sequence | TCGAATGCCTGGTGTAGCTG | TGCGATATCCACCGTAGTGC | Start: 1878 |
| Tm | 60.108 | 59.968 | End: 1992 |
| Size | 20 | 20 | 115 |
| Primer 3 details |
|---|
| Sequence | AAAAGCTCCTGGACCCGATG | CGTGACGTGATTCTCCTCCC | Start: 1023 |
| Tm | 60.035 | 60.179 | End: 1288 |
| Size | 20 | 20 | 266 |
| Primer 4 details |
|---|
| Sequence | AGGCAGATCCACCATTGACG | GAGGACATGCAGCCATCAGT | Start: 1322 |
| Tm | 60.108 | 60.107 | End: 1421 |
| Size | 20 | 20 | 100 |
| Primer 5 details |
|---|
| Sequence | AGGCAGATCCACCATTGACG | GTCTGAGGACATGCAGCCAT | Start: 1322 |
| Tm | 60.108 | 60.107 | End: 1425 |
| Size | 20 | 20 | 104 |
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