ssr_primer
Primer Details for TRINITY_DN77439_c4_g3_i1 |
|---|
| (CTTT)5: Primer 1 details |
|---|
| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | AATGCCGCGAATCTCTCTGT | GACGAGAGGATGCTCCGATG | Start: 155 |
| Tm | 59.823 | 60.04 | End: 376 |
| Size | 20 | 20 | 222 |
| Primer 2 details |
|---|
| Sequence | ACGTGTCCGGGAAAAACTGA | GACGAGAGGATGCTCCGATG | Start: 262 |
| Tm | 59.821 | 60.04 | End: 376 |
| Size | 20 | 20 | 115 |
| Primer 3 details |
|---|
| Sequence | AGTGAACGTGTCCGGGAAAA | GACGAGAGGATGCTCCGATG | Start: 257 |
| Tm | 59.821 | 60.04 | End: 376 |
| Size | 20 | 20 | 120 |
| Primer 4 details |
|---|
| Sequence | AATGCCGCGAATCTCTCTGT | GAGGATGCTCCGATGAAGCA | Start: 155 |
| Tm | 59.823 | 59.894 | End: 371 |
| Size | 20 | 20 | 217 |
| Primer 5 details |
|---|
| Sequence | ACGTGTCCGGGAAAAACTGA | GAGGATGCTCCGATGAAGCA | Start: 262 |
| Tm | 59.821 | 59.894 | End: 371 |
| Size | 20 | 20 | 110 |
(T)12: Primer 1 details | | Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | AATGCCGCGAATCTCTCTGT | GACGAGAGGATGCTCCGATG | Start: 155 |
| Tm | 59.823 | 60.04 | End: 376 |
| Size | 20 | 20 | 222 |
| Primer 2 details |
|---|
| Sequence | ACGTGTCCGGGAAAAACTGA | GACGAGAGGATGCTCCGATG | Start: 262 |
| Tm | 59.821 | 60.04 | End: 376 |
| Size | 20 | 20 | 115 |
| Primer 3 details |
|---|
| Sequence | AGTGAACGTGTCCGGGAAAA | GACGAGAGGATGCTCCGATG | Start: 257 |
| Tm | 59.821 | 60.04 | End: 376 |
| Size | 20 | 20 | 120 |
| Primer 4 details |
|---|
| Sequence | AATGCCGCGAATCTCTCTGT | GAGGATGCTCCGATGAAGCA | Start: 155 |
| Tm | 59.823 | 59.894 | End: 371 |
| Size | 20 | 20 | 217 |
| Primer 5 details |
|---|
| Sequence | ACGTGTCCGGGAAAAACTGA | GAGGATGCTCCGATGAAGCA | Start: 262 |
| Tm | 59.821 | 59.894 | End: 371 |
| Size | 20 | 20 | 110 |
(A)11: Primer 1 details | | Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | AATGCCGCGAATCTCTCTGT | GACGAGAGGATGCTCCGATG | Start: 155 |
| Tm | 59.823 | 60.04 | End: 376 |
| Size | 20 | 20 | 222 |
| Primer 2 details |
|---|
| Sequence | ACGTGTCCGGGAAAAACTGA | GACGAGAGGATGCTCCGATG | Start: 262 |
| Tm | 59.821 | 60.04 | End: 376 |
| Size | 20 | 20 | 115 |
| Primer 3 details |
|---|
| Sequence | AGTGAACGTGTCCGGGAAAA | GACGAGAGGATGCTCCGATG | Start: 257 |
| Tm | 59.821 | 60.04 | End: 376 |
| Size | 20 | 20 | 120 |
| Primer 4 details |
|---|
| Sequence | AATGCCGCGAATCTCTCTGT | GAGGATGCTCCGATGAAGCA | Start: 155 |
| Tm | 59.823 | 59.894 | End: 371 |
| Size | 20 | 20 | 217 |
| Primer 5 details |
|---|
| Sequence | ACGTGTCCGGGAAAAACTGA | GAGGATGCTCCGATGAAGCA | Start: 262 |
| Tm | 59.821 | 59.894 | End: 371 |
| Size | 20 | 20 | 110 |
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