Primer Details for TRINITY_DN77438_c1_g5_i1 |
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| (T)25: Primer 1 details |
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| Primer Characteristics | Forward Primer | Reverse Primer | Product Details |
| Sequence | TCCCTTTTTCCTAAAATGTGATAACA | TGTTAAAAAGGCACAAAGAGCA | Start: 0 |
| Tm | 57.286 | 57.272 | End: 102 |
| Size | 26 | 22 | 103 |
| Primer 2 details |
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| Sequence | CCCTTTTTCCTAAAATGTGATAACAGA | TGTTAAAAAGGCACAAAGAGCA | Start: 1 |
| Tm | 58.198 | 57.272 | End: 102 |
| Size | 27 | 22 | 102 |
| Primer 3 details |
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| Sequence | TCCCTTTTTCCTAAAATGTGATAACAG | TGTTAAAAAGGCACAAAGAGCA | Start: 0 |
| Tm | 58.198 | 57.272 | End: 102 |
| Size | 27 | 22 | 103 |
| Primer 4 details |
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| Sequence | CCCTTTTTCCTAAAATGTGATAACAG | TGTTAAAAAGGCACAAAGAGCA | Start: 1 |
| Tm | 57.014 | 57.272 | End: 102 |
| Size | 26 | 22 | 102 |
| Primer 5 details |
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| Sequence | TCCCTTTTTCCTAAAATGTGATAACA | TGTTAAAAAGGCACAAAGAGCAA | Start: 0 |
| Tm | 57.286 | 57.922 | End: 102 |
| Size | 26 | 23 | 103 |
